LEF1 anticorps (AA 257-270)

N° du produit ABIN297259

Détail du produit anti-LEF1 anticorps

Antigène: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Épitope: AA 257-270

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Cheval, Porc, Poisson zèbre (Danio rerio), Singe, Poulet, Roussette (Chauve-souris), Hamster

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp LEF1 est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Human LEF1.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Stickleback, Zebrafish (100%) Xenopus (93%) Pufferfish (86%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-QHEQRKEQEPKRPH from an internal region of human LEF1 (NP_057353.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Stickleback, Zebrafish (100%), Xenopus (93%), Pufferfish (86%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48 kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2 kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3,21(11):2703-14., PMID: 12032083). Recommended concentration: 1-3 μg/mL.

Not recommended for: IHC-P

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur LEF1

Antigène: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Autre désignation: LEF1 (LEF1 Produits)

Synonymes: anticorps LEF1, anticorps 3000002B05, anticorps AI451430, anticorps Lef-1, anticorps LEF-1, anticorps TCF10, anticorps TCF1ALPHA, anticorps TCF7L3, anticorps lymphoid enhancer binding factor 1, anticorps lymphoid enhancer-binding factor 1, anticorps lymphoid enhancer factor XLEF-1B, anticorps LEF1, anticorps lef1, anticorps Lef1

Sujet: Name/Gene ID: LEF1

Synonyms: LEF1, LEF-1, TCF7L3, TCF1-alpha, TCF10, TCF1ALPHA

ID gène: 51176

NCBI Accession: NP_057353

UniProt: Q9UJU2

Pathways: Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding