Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) anticorps (APC)

Détails pour le produit réf. ABIN301988
Antigène
  • CD11C
  • SLEB6
  • RGD1561123
  • AI449405
  • Cd11c
  • Cr4
  • N418
  • integrin subunit alpha X
  • integrin alpha X
  • ITGAX
  • Itgax
Reactivité
Humain, Singe
363
123
29
19
18
10
2
2
2
1
Hôte
Souris
268
108
52
37
7
3
Clonalité (Clone)
Monoclonal ()
Conjugué
APC
45
45
42
24
14
10
10
7
7
6
6
5
5
5
5
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
325
120
92
88
66
54
50
37
8
6
5
5
3
2
1
1
1
1
1
1
Options
Immunogène Dendritic cells of synovial fluid
Clone BU15
Isotype IgG1
Specificité The antibody BU15 reacts with CD11c (alphaX, p150), a 150 kDa integrin expressed mainly on dendritic cells and tissue macrophages.
HLDA III, WS Code M 256
HLDA V, WS Code AS S143
HLDA VI, WS Code AS Ref.6
Réactivité croisée (Details) Human, Monkey
Antigène
Autre désignation CD11c (ITGAX Antibody Extrait)
Sujet CD11c (p150, alphaX integrin subunit) forms complex with CD18 (beta2 integrin subunit) and is expressed mainly on tissue macrophages and dendritic cells. CD11c binds to complement fragment iC3b, fibrinogen, VCAM-1 and ICAM-2 or e.g. CD90. Like other beta2 integrins, CD11c/CD18 plays roles in cell migration and phagocytosis. Moreover, interaction of CD11c/CD18 with plasminogen regulates plasmin activities, and interaction with heparin counteracts binding of iC3b.
Pathways Activated T Cell Proliferation, Integrin Complex
Indications d'application The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Commentaires

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Do not freeze.
Avoid prolonged exposure to light.
Stock 4 °C
Stockage commentaire Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Images (Fournisseur)
Flow Cytometry (FACS) image for anti-Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) antibody (APC) (ABIN301988) Surface staining of human peripheral blood cells with anti-CD11c (BU15) PerCP (monocy...
Background publications Bullard, Hu, Adams, Schoeb, Barnum: "p150/95 (CD11c/CD18) expression is required for the development of experimental autoimmune encephalomyelitis." dans: The American journal of pathology, Vol. 170, Issue 6, pp. 2001-8, 2007 (PubMed).

Sadhu, Ting, Lipsky, Hensley, Garcia-Martinez, Simon, Staunton: "CD11c/CD18: novel ligands and a role in delayed-type hypersensitivity." dans: Journal of leukocyte biology, Vol. 81, Issue 6, pp. 1395-403, 2007 (PubMed).

Gang, Choi, Lee, Nham: "Identification of critical residues for plasminogen binding by the alphaX I-domain of the beta2 integrin, alphaXbeta2." dans: Molecules and cells, Vol. 24, Issue 2, pp. 240-6, 2007 (PubMed).

Vorup-Jensen, Chi, Gjelstrup, Jensen, Jewett, Xie, Shimaoka, Linhardt, Springer: "Binding between the integrin alphaXbeta2 (CD11c/CD18) and heparin." dans: The Journal of biological chemistry, Vol. 282, Issue 42, pp. 30869-77, 2007 (PubMed).

Produit citée dans: Angel, Lala, Chen, Edgar, Ostrovsky, Dunbar: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." dans: International immunology, Vol. 19, Issue 11, pp. 1271-9, 2007 (PubMed).

Myou, Zhu, Boetticher, Qin, Myo, Meliton, Lambertino, Munoz, Hamann, Leff: "Regulation of adhesion of AML14.3D10 cells by surface clustering of beta2-integrin caused by ERK-independent activation of cPLA2." dans: Immunology, Vol. 107, Issue 1, pp. 77-85, 2002 (PubMed).

Van der Vieren, Le Trong, Wood, Moore, St John, Staunton, Gallatin: "A novel leukointegrin, alpha d beta 2, binds preferentially to ICAM-3." dans: Immunity, Vol. 3, Issue 6, pp. 683-90, 1996 (PubMed).

Hogg, Takacs, Palmer, Selvendran, Allen: "The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules." dans: European journal of immunology, Vol. 16, Issue 3, pp. 240-8, 1986 (PubMed).

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