Souris anti-Humain IgG (Fc Region), (Heavy Chain) Anticorps

Détails pour le produit réf. ABIN302085, Fournisseur: Connectez-vous pour afficher
Antigène
Épitope
Fc Region, Heavy Chain
4782
2534
1960
1066
1022
861
306
292
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reactivité
Humain
2896
2761
2297
1676
1453
790
626
454
395
387
359
346
298
296
221
138
135
130
41
31
29
24
16
11
10
8
7
6
5
5
4
4
4
3
3
3
3
3
3
3
2
2
1
1
1
1
1
Hôte
Souris
6786
4909
1788
922
609
392
91
68
55
18
8
5
5
5
2
1
1
Clonalité (Clone)
Monoclonal ()
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
Options
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Immunogène Fusion protein of human IgG Fc fragment.
Clone EM-07
Isotype IgG1
Specificité The antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Réactivité croisée (Details) The antibody EM-07 does not crossreact with IgM.
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Pureté > 95 % (by SDS-PAGE)
Sujet Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Poids moléculaire 150 kDa
Indications d'application Western Blotting:
Application note: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by WB.
ELISA:
Application note: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by ELISA.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Agent conservateur Sodium azide
Précaution d'utilisation WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Conseil sur la manipulation Do not freeze.
Stock 4 °C
Stockage commentaire Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Western Blotting (WB) image for Mouse anti-Human IgG (Fc Region), (Heavy Chain) antibody (ABIN302085) IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purifi...
Background publications Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." dans: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." dans: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Franklin: "Structure and function of immunoglobulins." dans: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." dans: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).