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VCP anticorps

VCP Reactivité: Humain, Souris WB, ICC, FACS Hôte: Souris Monoclonal Hs-14 unconjugated
N° du produit ABIN302102
  • Antigène Voir toutes VCP Anticorps
    VCP (Valosin Containing Protein (VCP))
    Reactivité
    • 97
    • 48
    • 41
    • 15
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain, Souris
    Hôte
    • 81
    • 18
    • 2
    Souris
    Clonalité
    • 72
    • 29
    Monoclonal
    Conjugué
    • 60
    • 8
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    Cet anticorp VCP est non-conjugé
    Application
    • 68
    • 39
    • 26
    • 21
    • 21
    • 13
    • 13
    • 13
    • 8
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Specificité
    The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.
    Réactivité croisée (Details)
    Mouse, Human
    Purification
    Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
    Pureté
    > 95 % (by SDS-PAGE)
    Immunogène
    Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
    Clone
    Hs-14
    Isotype
    IgM
    Top Product
    Discover our top product VCP Anticorps primaire
  • Indications d'application
    Immunocytochemistry: Recommended dilution: 10 μg/mL, membrane permeabilization (acetone) is essential. The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought).
    Flow cytometry: Intraacrosomal staining, recommended dilution: 3-12 μg/mL.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Do not freeze.
  • Peknicova, Chladek, Hozak: "Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis." dans: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 53, Issue 1, pp. 42-9, (2005) (PubMed).

    Chládek, P?knicová, Capková, Geussová, Teplá, Madar: "[Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization]." dans: Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, Vol. 65, Issue 1, pp. 28-32, (2000) (PubMed).

  • Antigène
    VCP (Valosin Containing Protein (VCP))
    Autre désignation
    VCP (VCP Produits)
    Synonymes
    anticorps ALS14, anticorps IBMPFD, anticorps TERA, anticorps p97, anticorps CDC48, anticorps wu:fd16d05, anticorps wu:fj63d11, anticorps p93, anticorps 3110001E05, anticorps p97/VCP, anticorps 22.26, anticorps 7.12, anticorps Apple, anticorps BcDNA.GM02885, anticorps BcDNA:GM02885, anticorps CG2331, anticorps DmTER94, anticorps Dmel\CG2331, anticorps I, anticorps Ter94, anticorps Tera, anticorps VCP, anticorps VCP ATPase, anticorps VCP/p97, anticorps Vcp, anticorps anon-WO2004063362.65, anticorps anon-WO2004063362.67, anticorps cdc48, anticorps dTERA, anticorps dVCP, anticorps dmTERA, anticorps dvcp, anticorps l(2)03775, anticorps l(2)46CFf, anticorps l(2)46CFs, anticorps l(2)46Ch, anticorps pTER94, anticorps ter94, anticorps vcp, anticorps FANCG, anticorps valosin containing protein, anticorps valosin containing protein S homeolog, anticorps valosin-containing protein, anticorps CG2331 gene product from transcript CG2331-RA, anticorps zgc:136908, anticorps transitional endoplasmic reticulum ATPase, anticorps VCP, anticorps vcp.S, anticorps vcp, anticorps Vcp, anticorps Bm1_56585, anticorps LOC100136246, anticorps TER94, anticorps zgc:136908, anticorps LOC101109680
    Sujet
    Valosin containing protein,VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,TERA, CDC48
    ID gène
    7415
    UniProt
    P55072
    Pathways
    ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway
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