Cyclin B1 anticorps (CCNB1)

Details for Product anti-CCNB1 Antibody No. ABIN3022807
Antigène
  • ccnb
  • cycb
  • cb267
  • cycb1
  • wu:fa19g04
  • wu:fb16d01
  • wu:fb16e07
  • wu:fi21c01
  • ccnb1
  • MGC53596
  • CCNB
  • Ccnb1-rs1
  • Ccnb1-rs13
  • CycB1
  • Cycb-4
  • Cycb-5
  • Cycb1-rs1
  • cyclin B1
  • cyclin B1 S homeolog
  • ccnb1
  • ccnb1.2
  • ccnb1.S
  • CCNB1
  • Ccnb1
  • ccnb1.2.S
Reactivité
Souris, Rat (Rattus), Humain
474
237
151
52
10
8
8
6
5
4
3
3
1
1
1
Hôte
Lapin
343
148
1
Clonalité
Polyclonal
Conjugué
Cet anticorp Cyclin B1 est non-conjugé
18
11
11
11
11
11
11
11
9
7
6
6
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
273
132
131
111
93
75
68
52
22
21
21
16
14
11
8
3
3
1
Options
Immunogène A synthetic peptide of human CCNB1
Isotype IgG
Purification Affinity purification
Plasmids, Primers & others Plasmids, Primers & others Cyclin B1 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation CCNB1 (CCNB1 Antibody Extrait)
Sujet Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C. Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis. While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147. At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression. Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers.
Synonyms: CCNB1, CCNB, cyclin B1
ID gène 891
UniProt P14635
Pathways Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
Indications d'application Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze / thaw cycles
Stock -20 °C/-80 °C
Images (Fournisseur)
Western Blotting (WB) image for anti-Cyclin B1 (CCNB1) antibody (ABIN3022807) Western blot analysis of extracts of various cell lines, using CCNB1 antibody.
Immunofluorescence (IF) image for anti-Cyclin B1 (CCNB1) antibody (ABIN3022807) Immunofluorescence analysis of A549 cell using CCNB1 antibody. Blue: DAPI for nuclear...
Produit citée dans: Dong, Zheng, Wang, Tang, Yan, Zhou, Huang: "A novel HDAC6 inhibitor exerts an anti-cancer effect by triggering cell cycle arrest and apoptosis in gastric cancer." dans: European journal of pharmacology, Vol. 828, pp. 67-79, 2018 (PubMed). Méthode utilisée: Western Blotting (WB)

Deng, Li, Zhao, Yuan, Li: "Antitumor activity of the microtubule inhibitor MBRI-001 against human hepatocellular carcinoma as monotherapy or in combination with sorafenib." dans: Cancer chemotherapy and pharmacology, Vol. 81, Issue 5, pp. 853-862, 2018 (PubMed). Méthode utilisée: Western Blotting (WB)

Tsai, Yu, Lin, Cheng, Chen, Pang: "Prevention of Simvastatin-Induced Inhibition of Tendon Cell Proliferation and Cell Cycle Progression by Geranylgeranyl Pyrophosphate." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 149, Issue 2, pp. 326-34, 2016 (PubMed). Méthode utilisée: Western Blotting (WB)

Cheng, Yang, Fang, Ma, Gong, Wang, Li, Xu: "Molecular mechanism for USP7-mediated DNMT1 stabilization by acetylation." dans: Nature communications, Vol. 6, pp. 7023, 2015 (PubMed). Méthode utilisée: Western Blotting (WB)

Yu, Pang, Wu, Lin, Tseng, Tsai: "Platelet-rich plasma increases proliferation of tendon cells by modulating Stat3 and p27 to up-regulate expression of cyclins and cyclin-dependent kinases." dans: Cell proliferation, Vol. 48, Issue 4, pp. 413-20, 2015 (PubMed). Méthode utilisée: Western Blotting (WB)

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