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Histone 3 (H3) (H3K4me) anticorps Primary Antibody

H3 Reactivité: Humain, Souris, Rat ChIP, IF, IP, IHC, WB Hôte: Lapin Polyclonal
Pubmed (3)
N° du produit ABIN3023251
$490.05
Plus shipping costs $45.00
local_shipping Destination: Etats-Unis
Envoi sous 13 à 14 jours ouvrables
  • Antigène
    Épitope
    H3K4me
    Reactivité
    Humain, Souris, Rat
    Hôte
    Lapin
    Clonalité
    Polyclonal
    Conjugué
    Inconjugué
    Application
    Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
    Purification
    Affinity purification
    Immunogène
    A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
    Isotype
    IgG
  • Indications d'application
    Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:200, IP: 1:50-1:200, ChIP: 1:50-1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid freeze / thaw cycles
    Stock
    -20 °C/-80 °C
  • Zhang, Zhang, Cheng, Liu, Lin, Wu, Zhang, Wang, Wang, Guo, Zhang, Lei, Zhao, Zhu, Wan: "Functional characterization of rice CW-domain containing zinc finger proteins involved in histone recognition." dans: Plant science : an international journal of experimental plant biology, Vol. 263, pp. 168-176, 2018 (PubMed). Méthode utilisée: Chromatin Immunoprecipitation (ChIP)

    Chen, Zhang, Li, Feng, Zhang, Yao, Zhang, Wan: "Celastrol attenuates incision-induced inflammation and pain associated with inhibition of the NF-κB signalling pathway via SARM." dans: Life sciences, Vol. 205, pp. 136-144, 2018 (PubMed). Méthode utilisée: Western Blotting (WB)

    Cao, Liu, Yue, Liu, Pei, Gu, Wang, Jia: "Iron chelation inhibits cancer cell growth and modulates global histone methylation status in colorectal cancer." dans: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, Vol. 31, Issue 5, pp. 797-805, 2018 (PubMed). Méthode utilisée: Western Blotting (WB)

  • Antigène
    Autre désignation
    Histone H3
    Sujet
    Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.
    Synonyms: HIST1H3J , H3/j , H3FJ , Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h , Histone H3/I, Histone H3/j , Histone H3/k, Histone H3/l, HIST3H3
    ID gène
    8290
    UniProt
    Q16695
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