Actin anticorps

N° du produit ABIN3024131

Détail du produit anti-Actin anticorps

Antigène: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Actin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: Protein G affinity chromatography

Immunogène: An amino acid sequence common among human muscle actins was used as the immunogen for the Muscle Actin antibody.

Clone: PMAC1-1

Isotype: IgG1 kappa

Information d'application

Indications d'application: Titering of the Muscle Actin antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Actin

Antigène: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Autre désignation: Muscle Actin (ACTA1 Produits)

Synonymes: anticorps ACTA, anticorps ASMA, anticorps CFTD, anticorps CFTD1, anticorps CFTDM, anticorps MPFD, anticorps NEM1, anticorps NEM2, anticorps NEM3, anticorps acta, anticorps asma, anticorps cftd, anticorps cftd1, anticorps cftdm, anticorps mpfd, anticorps nem1, anticorps AA959943, anticorps Acta-2, anticorps Acts, anticorps Actsk-1, anticorps actc, anticorps actc1, anticorps cfk, anticorps hm:zeh0631, anticorps zeh0631, anticorps acta1, anticorps hm:zeh0293, anticorps zgc:112098, anticorps actin, alpha 1, skeletal muscle, anticorps actin, alpha 1, skeletal muscle L homeolog, anticorps actin, alpha 1b, skeletal muscle, anticorps actin, muscle, anticorps actin, alpha skeletal muscle, anticorps actin, alpha 1a, skeletal muscle, anticorps ACTA1, anticorps acta1.L, anticorps acta1, anticorps Acta1, anticorps acta1b, anticorps LOC100533357, anticorps LOC109479779, anticorps LOC109108280, anticorps acta1a

Sujet: Actin participates in many important cellular processes, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. An actin protein's mass is roughly 42-  kDa and it is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. It can be present as either a free monomer called G-actin (globular) or as part of a linear polymer microfilament called F-actin (filamentous), both of which are essential for such important cellular functions as the mobility and contraction of cells during cell division. [Wiki]

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells.

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development