MAP3K1 anticorps (AA 1211-1310)

N° du produit ABIN3025244

Détail du produit anti-MAP3K1 anticorps

Antigène: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Épitope: AA 1211-1310

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAP3K1 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogène: A partial recombinant protein (aa 1211-1310) was used as the immunogen for the MAP3K1 antibody.

Clone: 2F6

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal dilution of the MAP3K1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Prediluted IHC only format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the MAP3K1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur MAP3K1

Antigène: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Autre désignation: MAP3K1 (MAP3K1 Produits)

Synonymes: anticorps MAP3K1, anticorps Mekk1, anticorps MAPKKK1, anticorps MEKK1, anticorps Mekk, anticorps ARAKIN, anticorps ATMEKK1, anticorps MAPK/ERK kinase kinase 1, anticorps MAPKKK8, anticorps T15F16.5, anticorps T15F16_5, anticorps MEKK, anticorps MEKK 1, anticorps SRXY6, anticorps mitogen-activated protein kinase kinase kinase 1, anticorps mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase, anticorps MAPK/ERK kinase kinase 1, anticorps MAP3K1, anticorps map3k1, anticorps Map3k1, anticorps MEKK1

Sujet: Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli, including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (MEK kinase- 5). MEK kinase-1 activates the ERK and c-Jun NH2-terminal kinase (JNK) pathways by phosphorylation of MAP2K1 and MAP2K4, and also activates the central protein kinases of the NF-kB pathway, CHUK and IKBKB. Additionally, MEK kinase-1 uses an E3 ligase through its PHD domain, a RING-finger-like structure, to target proteins for degradation through ubiquitination.

Pathways: Signalisation MAPK, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades