S100A8/A9 Complex (Calprotectin) anticorps

N° du produit ABIN3026084

Détail du produit anti-S100A8/A9 Complex (Calprotectin) anticorps

Antigène: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))

Reactivité: Humain, Boeuf (Vache), Porc, Cat, Chien, Cobaye, Cheval, Singe, Lapin, Rat, Babouin, Chévre, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp S100A8/A9 Complex (Calprotectin) est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogène: An affinity purified monocyte membrane preparation was used as the immunogen for the MRP8 + MPR14 antibody.

Clone: MAC387

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the MRP8 + MPR14 antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the MRP8 + MPR14 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur S100A8/A9 Complex (Calprotectin)

Antigène: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))

Autre désignation: MRP8 + MPR14 (S100A8/A9 Produits)

Synonymes: anticorps 60B8AG, anticorps CAGB, anticorps CFAG, anticorps CGLB, anticorps L1AG, anticorps LIAG, anticorps MAC387, anticorps MIF, anticorps MRP14, anticorps NIF, anticorps P14, anticorps S100 calcium binding protein A9, anticorps S100A9

Sujet: Recognizes S100A8/MPR8 and S100A9/MRP14, an intra-cytoplasmic antigen comprising of a 12  kDa alpha chain and a 14  kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This mAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

Pathways: S100 Proteins