MUC5AC anticorps

N° du produit ABIN3026774

Détail du produit anti-MUC5AC anticorps

Antigène: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reactivité: Humain, Souris, Rat, Singe, Cat, Lapin, Poulet, Porc, Hedgehog

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MUC5AC est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aucune reactivité croisée: Boeuf (Vache)

Attributs du produit: This antibody recognizes the peptide core of gastric mucin M1 (>1,000 kDa), now identified as Mucin-5AC or MUC5AC. The MUC5AC epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. MUC5AC antibody reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

Purification: Protein G affinity chromatography

Immunogène: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for this MUC5AC antibody.

Clone: 45M1

Isotype: IgG1 kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC5AC antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the MUC5AC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur MUC5AC

Antigène: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Autre désignation: MUC5AC Mucin-5AC (MUC5AC Produits)

Synonymes: anticorps MUC5, anticorps TBM, anticorps leB, anticorps 2210005L13Rik, anticorps MGM, anticorps mucin 5AC, oligomeric mucus/gel-forming, anticorps putative gastric mucin, anticorps mucin 5, subtypes A and C, tracheobronchial/gastric, anticorps mucin 2, oligomeric mucus/gel-forming, anticorps MUC5AC, anticorps Smp_144070, anticorps Muc5ac, anticorps MUC2

Sujet: This antibody recognizes the peptide core of gastric mucin M1 (>1,000  kDa), now identified as Mucin-5AC or MUC5AC. The MUC5AC epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. MUC5AC antibody reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

ID gène: 4586