Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
Purification
Antigen affinity
Immunogène
An amino acid sequence from the middle region of CaMKK2 (KLAYNENDNTYYAMK) was used as the immunogen for this CaMKK2 antibody (100% homologous in human, mouse and rat).
CAMKK2
Reactivité: Humain
WB, ELISA, IHC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
The stated application concentrations are suggested starting amounts. Titration of the CaMKK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Stock
-20 °C
Stockage commentaire
After reconstitution, the CaMKK2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Antigène
CAMKK2
(Calcium/calmodulin-Dependent Protein Kinase Kinase 2, beta (CAMKK2))
anticorps CAMKK, anticorps CAMKKB, anticorps 6330570N16Rik, anticorps AW061083, anticorps mKIAA0787, anticorps camk2b, anticorps fj90d04, anticorps wu:fj90d04, anticorps si:dkey-174n20.2, anticorps CAMKK2, anticorps calcium/calmodulin dependent protein kinase kinase 2, anticorps calcium/calmodulin-dependent protein kinase kinase 2, beta, anticorps calcium/calmodulin-dependent protein kinase (CaM kinase) II beta 1, anticorps calcium/calmodulin dependent protein kinase II beta, anticorps calcium/calmodulin-dependent protein kinase kinase 2, anticorps CAMKK2, anticorps Camkk2, anticorps camk2b1, anticorps CAMK2B, anticorps camkk2
Sujet
Calcium/calmodulin-dependent protein kinase kinase 2, is an enzyme that in humans is encoded by the CaMKK2 gene. The product of this gene belongs to the serine/threonine-specific protein kinase family, and to the Ca++/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent(CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. CaMKK2 regulates production of the appetite stimulating hormone neuropeptide Y and functions as an AMPK kinase in the hypothalamus. It also has an important role in the development of hyperalgesia and tolerance to opioid analgesic drugs, through reduction in downstream signalling pathways and mu opioid receptor downregulation. Inhibition of CaMKK2 in mice reduces appetite and promotes weight loss.