The stated application concentrations are suggested starting amounts. Titration of the HIF-3 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Stock
-20 °C
Stockage commentaire
After reconstitution, the HIF-3 alpha antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. This gene involved in adaptive response to hypoxia. HIF3a suppresses hypoxia-inducible expression of < a href=../search_result.php?search_txt=hif1a>HIF1a and < a href=../search_result.php?search_txt=hif2a>HIF2a. It binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element(HRE) of target gene promoters. The complex HIF3a-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.