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SMAD3 anticorps (pSer423, pSer425)

SMAD3 Reactivité: Humain, Souris, Rat, Porc, Poulet, Poisson zèbre (Danio rerio), Boeuf (Vache), Grenouille WB, IHC (p), IF, EIA Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN303328
  • Antigène Voir toutes SMAD3 Anticorps
    SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
    Épitope
    • 34
    • 29
    • 28
    • 28
    • 20
    • 14
    • 14
    • 11
    • 11
    • 9
    • 8
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 417-425, pSer423, pSer425
    Reactivité
    • 257
    • 150
    • 138
    • 28
    • 10
    • 9
    • 8
    • 8
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Humain, Souris, Rat, Porc, Poulet, Poisson zèbre (Danio rerio), Boeuf (Vache), Grenouille
    Hôte
    • 248
    • 23
    Lapin
    Clonalité
    • 231
    • 40
    Polyclonal
    Conjugué
    • 138
    • 19
    • 14
    • 14
    • 12
    • 8
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp SMAD3 est non-conjugé
    Application
    • 231
    • 117
    • 108
    • 74
    • 59
    • 55
    • 55
    • 53
    • 28
    • 13
    • 10
    • 8
    • 5
    • 4
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
    Specificité
    This antibody recognises SMAD3 pSer423/425.
    Réactivité croisée (Details)
    Species reactivity (expected):Bovine, Chicken, Frog, Mouse, Pig, Rat and Zebrafish.
    Species reactivity (tested):Human.
    Purification
    Immunoaffinity Chromatography.
    Immunogène
    Synthetic peptide corresponding to amino acids 417-425 of Human SMAD3 protein.
    Top Product
    Discover our top product SMAD3 Anticorps primaire
  • Indications d'application
    ELISA (1/25000-1/100000). Western Blot (1/2000-1/20000). Immunofluorescence. Immunohistochemistry on Paraffin Sections (2.5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Sun, Zhou, Gui, Shimokado, Nakanishi, Oikawa, Sato, Muragaki: "Elevated serum 1,25(OH)2-vitamin D3 level attenuates renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction in kl/kl mice." dans: Scientific reports, Vol. 4, pp. 6563, (2014) (PubMed).

    Carracedo, Lu, Popova, Jonsson, Eckes, Gullberg: "The fibroblast integrin alpha11beta1 is induced in a mechanosensitive manner involving activin A and regulates myofibroblast differentiation." dans: The Journal of biological chemistry, Vol. 285, Issue 14, pp. 10434-45, (2010) (PubMed).

    Liu, Carson-Walter, Cooper, Winans, Johnson, Walter: "Vascular gene expression patterns are conserved in primary and metastatic brain tumors." dans: Journal of neuro-oncology, Vol. 99, Issue 1, pp. 13-24, (2010) (PubMed).

  • Antigène
    SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
    Autre désignation
    SMAD3 (SMAD3 Produits)
    Synonymes
    anticorps HSPC193, anticorps HsT17436, anticorps JV15-2, anticorps LDS1C, anticorps LDS3, anticorps MADH3, anticorps madh3, anticorps madh3a, anticorps smad3, anticorps wu:fa99e03, anticorps XSmad3, anticorps XenMLP, anticorps Xmad3, anticorps madh3-A, anticorps Madh3, anticorps AU022421, anticorps Smad 3, anticorps mad3, anticorps madh3b, anticorps zgc:92234, anticorps SMAD family member 3, anticorps SMAD family member 3a, anticorps SMAD family member 3 L homeolog, anticorps SMAD family member 3b, anticorps SMAD3, anticorps smad3a, anticorps smad3.L, anticorps Smad3, anticorps smad3b
    Sujet
    Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-b-mediated response.Synonyms: JV15-2, MAD homolog 3, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, SMAD family member 3, SMAD-3
    ID gène
    4088
    NCBI Accession
    NP_001138574
    UniProt
    P84022
    Pathways
    Cycle Cellulaire, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
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