Species reactivity (expected):Mammal and Rabbit. Species reactivity (tested):Human.
Purification
Immunoaffinity Chromatography.
Immunogène
KLH conjugated Synthetic Peptide corresponding to aa 286-293 (of 390) of full-length (including signal peptide) precursor TGFb protein. Genename: TGFB1
TGFB1
Reactivité: Humain, Souris, Rat
IHC, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: 1/2000-1/10000. Western Blot: 1/200-1/1000. Immunofluorescence. Immunohistochemistry on Paraffin Sections: 5 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % Sodium Azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, which includes three mammalian TGF-beta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins. The most abundant isoform, TGF-beta-1, is a 25 kDa homodimer composed of two 12.5 kDa subunits joined by disulfide bonds. TGF-beta-1 is a highly conserved molecule - the amino acid sequence between human and mouse differ only by one residue. Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types. It is produced by a wide variety of cell and tissue types during all stages of cell differentiation. TGF-beta-1 sources include platelets, bone and soft tissues such as placenta and kidneys.Synonyms: TGF-beta-1, TGFB, Transforming growth factor beta-1