PTTG1 anticorps (C-Term)

N° du produit ABIN3042643

Détail du produit anti-PTTG1 anticorps

Antigène: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Épitope: AA 146-161, C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PTTG1 est non-conjugé

Application: Western Blotting (WB)

Fonction: Rabbit IgG polyclonal antibody for Securin(PTTG1) detection. Tested with WB in Human.

Séquence: LMILDEEREL EKLFQL

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Securin(PTTG1) detection. Tested with WB in Human.
Gene Name: pituitary tumor-transforming 1
Protein Name: Securin

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human Securin(146-161aa LMILDEERELEKLFQL), different from the related rat sequence by four amino acids, and from the related mouse sequence by five amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Références pour anticorps anti-PTTG1 (ABIN3042643)

Zhang, Liu, Xu, Huang, Tan, Sun, Lu: "Pituitary tumor-transforming gene 1 (PTTG1) is overexpressed in oral squamous cell carcinoma (OSCC) and promotes migration, invasion and epithelial-mesenchymal transition (EMT) in SCC15 cells." dans: Tumour biology, Vol. 35, Issue 9, pp. 8801-11, (2014) (PubMed).

Détails sur PTTG1

Antigène: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Autre désignation: PTTG1 (PTTG1 Produits)

Synonymes: anticorps EAP1, anticorps HPTTG, anticorps PTTG, anticorps TUTR1, anticorps PTTG1, anticorps securin, anticorps AW555095, anticorps C87862, anticorps Pttg, anticorps Pttg3, anticorps Securin, anticorps Protein Cut2, anticorps pituitary tumor-transforming 1, anticorps pituitary tumor-transforming gene 1, anticorps securin, sister chromatid separation inhibitor, anticorps PTTG1, anticorps Pttg1, anticorps cut2

Sujet: Securin, also known as pituitary tumor-transforming 1 or EAP1 is a protein that in humans is encoded by the PTTG1 gene. PTTG1 gene is mapped to 5q33.3. The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex(APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box(D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.

Synonyms: EAP1 antibody|PTTG antibody|TUTR1 antibody|Hpttg antibody|TUMOR-TRANSFORMING 1 antibody|Esp1-associated protein antibody|ESP1-ASSOCIATED PROTEIN 1 antibody|Tumor-transforming protein 1 antibody|Pituitary tumor-transforming gene 1 protein antibody

UniProt: O95997