CYP1A2 anticorps (AA 185-320)

N° du produit ABIN3043820

Détail du produit anti-CYP1A2 anticorps

Antigène: CYP1A2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2))

Épitope: AA 185-320

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CYP1A2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Cytochrome P450 1A2(CYP1A2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Cytochrome P450 1A2(CYP1A2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: cytochrome P450, family 1, subfamily A, polypeptide 2
Protein Name: Cytochrome P450 1A2

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human CYP1A2 recombinant protein (Position: H185-D320). Human CYP1A2 shares 69.9% and 72.1% amino acid (aa) sequence identity with mouse and rat CYP1A2, respectively.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-CYP1A2 (ABIN3043820)

Fan, Jiang, Wang, Tan, Zeng, Wang, Chen, Qu, Gonzalez, Huang, Bi: "Wuzhi tablet (Schisandra Sphenanthera extract) protects against acetaminophen-induced hepatotoxicity by inhibition of CYP-mediated bioactivation and regulation of NRF2-ARE and p53/p21 pathways." dans: Drug metabolism and disposition: the biological fate of chemicals, Vol. 42, Issue 12, pp. 1982-90, (2014) (PubMed).

Jiang, Fan, Wang, Chen, Zeng, Tan, Gonzalez, Huang, Bi: "Schisandrol B protects against acetaminophen-induced hepatotoxicity by inhibition of CYP-mediated bioactivation and regulation of liver regeneration." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 143, Issue 1, pp. 107-15, (2014) (PubMed).

Détails sur CYP1A2

Antigène: CYP1A2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2))

Autre désignation: CYP1A2 (CYP1A2 Produits)

Synonymes: anticorps CP12, anticorps P3-450, anticorps P450(PA), anticorps CYP1A2, anticorps CYPIA2, anticorps P450-PM4, anticorps Cyp1a1, anticorps P450-3, anticorps CYPD45, anticorps P-450d, anticorps RATCYPD45, anticorps CYP1A5, anticorps CP450, anticorps CYP-IA2, anticorps cytochrome P450 family 1 subfamily A member 2, anticorps cytochrome P450 1A2, anticorps cytochrome P450 LM4, anticorps cytochrome P450, family 1, subfamily A, polypeptide 2, anticorps cytochrome P450, family 1, subfamily a, polypeptide 2, anticorps cytochrome P450 1A2-like, anticorps CYP1A2, anticorps LOC100328937, anticorps Cyp1a2, anticorps VDBG_06455, anticorps LOC101825565, anticorps LOC102130662

Sujet: CYP1A2 (Cytochrome P450, Subfamily I, Polypeptide 2) is a member of the cytochrome P450 mixed-function oxidase system and is involved in the metabolism of xenobiotics in the body. CYP1A2 is a member of the cytochrome P450 superfamily of enzymes. In humans, the CYP1A2 enzyme is encoded by the CYP1A2 gene. CYP1A2 localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The CYP1A2 gene encodes a P450 enzyme involved in O-deethylation of phenacetin. Ikeya et al. (1989) found that the human CYP1A2 gene spans almost 7.8 kb and contains 7 exons. The CYP1A2 gene is mapped on 15q24.1. CYP1A2 accounts for nearly 15 % of the cytochrome P450 in the human liver. CYP1A2 displays higher activity in men than in women, and is inhibited by oral contraceptives. Inducers of CYP1A2 include cruciferous vegetables. Cigarette smoking has also been shown to increase CYP1A2 activity. Expression of CYP1A2 appears to be induced by various dietary constituents.

Synonyms: Aryl hydrocarbon hydroxylase antibody|CP 12 antibody|CP12 antibody|CP1A2_HUMAN antibody| CYP1A2 antibody|CYPIA2 antibody|Cytochrome P(3)450 antibody|Cytochrome P450 1A2 antibody| Cytochrome P450 4 antibody|Cytochrome P450 family 1 polypeptide 2 antibody|Cytochrome P450 family 1 subfamily A polypeptide 2 antibody|Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2 antibody|Cytochrome P450-P3 antibody|Cytochrome P4501A2 antibody| Dioxin inducable P3 450 antibody|Flavoprotein linked monooxygenase antibody|Microsomal monooxygenase antibody|P(3)450 antibody|P3 450 antibody|P450 4 antibody|P450 form 4 antibody|P450 P3 antibody|P450(PA) antibody|Xenobiotic monooxygenase antibody

ID gène: 1544

UniProt: P05177