HIF1A anticorps (C-Term)

N° du produit ABIN3043841

Détail du produit anti-HIF1A anticorps

Antigène: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Épitope: AA 703-732, C-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HIF1A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Hypoxia-inducible factor 1-alpha(HIF1A) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Séquence: EEELNPKILA LQNAQRKRKM EHDGSLFQAV

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Hypoxia-inducible factor 1-alpha(HIF1A) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Protein Name: Hypoxia-inducible factor 1-alpha

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminal of human HIF-1-alpha (703-732aa EEELNPKILALQNAQRKRKMEHDGSLFQAV), different from the related mouse and rat sequences by three amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, The detection limit for HIF-1-alpha is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-HIF1A (ABIN3043841)

Wang, Yuan, Wang, Yang, Chen, Liu, Song, Feng, Tan, Jia: "Anti-inflammatory Effects of Phyllanthus emblica L on Benzopyrene-Induced Precancerous Lung Lesion by Regulating the IL-1β/miR-101/Lin28B Signaling Pathway." dans: Integrative cancer therapies, Vol. 16, Issue 4, pp. 505-515, (2018) (PubMed).

Qin, Ke, Zhou, Wang, Liang, Wang, Yang, Gao, Ye, Kumar, Wang: "Metastasis-Associated Protein 1 Deficiency Results in Compromised Pulmonary Alveolar Capillary Angiogenesis in Mice." dans: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 3932-3941, (2018) (PubMed).

Yang, Chen, Wang, Xian, Zhang, Zhu, Pan, Cheng: "TLR4 promotes the expression of HIF-1α by triggering reactive oxygen species in cervical cancer cells in vitro-implications for therapeutic intervention." dans: Molecular medicine reports, Vol. 17, Issue 2, pp. 2229-2238, (2018) (PubMed).

Guo, Mu, Yu, Li, Chen, Wei, Bi, Yu, Liang: "Comparison of the expression of TGF-β1, E-cadherin, N-cadherin, TP53, RB1CC1 and HIF-1α in oral squamous cell carcinoma and lymph node metastases of humans and mice." dans: Oncology letters, Vol. 15, Issue 2, pp. 1639-1645, (2018) (PubMed).

Gao, Chen, Gao, Wang, Sun, Yang: "CD47 deficiency in tumor stroma promotes tumor progression by enhancing angiogenesis." dans: Oncotarget, Vol. 8, Issue 14, pp. 22406-22413, (2017) (PubMed).

Wan, Wu: "Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways." dans: Journal of experimental & clinical cancer research : CR, Vol. 35, Issue 1, pp. 119, (2017) (PubMed).

Li, Shi, Huang, Wang, Yu, Guo, Ren: "Suppression of the expression of hypoxia-inducible factor-1α by RNA interference alleviates hypoxia-induced pulmonary hypertension in adult rats." dans: International journal of molecular medicine, Vol. 38, Issue 6, pp. 1786-1794, (2017) (PubMed).

Liu, Murray-Stewart, Casero, Kagiampakis, Jin, Zhang, Wang, Che, Tong, Ke, Jiang, Wang, Wan: "Targeting hexokinase 2 inhibition promotes radiosensitization in HPV16 E7-induced cervical cancer and suppresses tumor growth." dans: International journal of oncology, Vol. 50, Issue 6, pp. 2011-2023, (2017) (PubMed).

Ding, Chen, Xu, Qin: "Effect of Hyperbaric Oxygen on the Growth of Intracranial Glioma in Rats." dans: Chinese medical journal, Vol. 128, Issue 23, pp. 3197-203, (2016) (PubMed).

Wan, Wu, Zhang: "Local recurrence of small cell lung cancer following radiofrequency ablation is induced by HIF-1α expression in the transition zone." dans: Oncology reports, Vol. 35, Issue 3, pp. 1297-308, (2016) (PubMed).

Wang, Fang, Tan, Zhang, Ma: "Hypoxic preconditioning increases the protective effect of bone marrow mesenchymal stem cells on spinal cord ischemia/reperfusion injury." dans: Molecular medicine reports, Vol. 13, Issue 3, pp. 1953-60, (2016) (PubMed).

Wu, Ye, Deng, Wu, Li, Zhang: "Upregulation of metastasis-associated gene 2 promotes cell proliferation and invasion in nasopharyngeal carcinoma." dans: OncoTargets and therapy, Vol. 9, pp. 1647-56, (2016) (PubMed).

Xu, Dong: "Role of hypoxia-inducible factor-1α in pathogenesis and disease evaluation of ulcerative colitis." dans: Experimental and therapeutic medicine, Vol. 11, Issue 4, pp. 1330-1334, (2016) (PubMed).

Ren, Wang, Xu, Dai: "Effect of rapamycin on endometriosis in mice." dans: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 101-106, (2016) (PubMed).

Sun, He, Geng: "Neuroprotective mechanism of HIF-1α overexpression in the early stage of acute cerebral infarction in rats." dans: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 391-395, (2016) (PubMed).

Dai, Sun, Yao, Chen, Yu, Long: "Exposure to concentrated ambient fine particulate matter disrupts vascular endothelial cell barrier function via the IL-6/HIF-1α signaling pathway." dans: FEBS open bio, Vol. 6, Issue 7, pp. 720-8, (2016) (PubMed).

Wang, Jing, Shan, Chen, Guo, Cao, Mu, Peng, Zhou, Li: "Semaphorin 4D and hypoxia-inducible factor-1α overexpression is related to prognosis in colorectal carcinoma." dans: World journal of gastroenterology, Vol. 21, Issue 7, pp. 2191-8, (2015) (PubMed).

Wang, Wu, Guo, Zhang, Zhang, Zhan: "Increased hypoxia-inducible factor 1alpha expression in rat brain tissues in response to aging." dans: Neural regeneration research, Vol. 7, Issue 10, pp. 778-82, (2015) (PubMed).

Wu, Wang, Zhang, Zhang, Zhang, Zhang: "Puerarin decreases hypoxia inducible factor-1 alpha in the hippocampus of vascular dementia rats." dans: Neural regeneration research, Vol. 7, Issue 6, pp. 421-5, (2015) (PubMed).

Feng, Tao, Dawei, Xuliang, Xiaodong: "HIF-1? expression correlates with cellular apoptosis, angiogenesis and clinical prognosis in rectal carcinoma." dans: Pathology oncology research : POR, Vol. 20, Issue 3, pp. 603-10, (2014) (PubMed).

Détails sur HIF1A

Antigène: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Autre désignation: HIF1A (HIF1A Produits)

Synonymes: anticorps hif1a, anticorps AA959795, anticorps HIF1alpha, anticorps MOP1, anticorps bHLHe78, anticorps HIF-1A, anticorps HIF-1alpha, anticorps HIF1, anticorps HIF1-ALPHA, anticorps PASD8, anticorps HIF1-alpha, anticorps hif-1a, anticorps hypoxia inducible factor 1 alpha, anticorps hypoxia inducible factor 1, alpha subunit, anticorps hypoxia inducible factor 1 alpha subunit, anticorps HIF (Hypoxia Inducible Factor) homologa, anticorps hif-1a, anticorps Hif1a, anticorps HIF1A, anticorps hif1a, anticorps hif-1

Sujet: HIF-1α (Hypoxia-inducible factor 1α, HIF1A) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including angiogenesis, erythropoiesis and glycolysis. This gene was mapped to 14q21-q24. HIF-1α transactivate genes required for energy metabolism and tissue perfusion and is necessary for embryonic development and tumor explant growth. HIF-1alpha is over expressed during carcinogenesis, myocardial infarction and wound healing. It is crucial for the cellular response to hypoxia and is frequently over expressed in human cancers, resulting in the activation of genes essential for cell survival. HIF-1α regulates the survival and function in the inflammatory microenvironment directly. It is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.

Synonyms: ARNT interacting protein antibody|ARNT-interacting protein antibody|Basic helix loop helix PAS protein MOP1 antibody|Basic-helix-loop-helix-PAS protein MOP1 antibody|bHLHe78 antibody|Class E basic helix-loop-helix protein 78 antibody|HIF 1A antibody|HIF 1alpha antibody|HIF-1-alpha antibody|HIF1 A antibody|HIF1 Alpha antibody|HIF1 antibody|HIF1-alpha antibody|HIF1A antibody|HIF1A_HUMAN antibody|Hypoxia inducible factor 1 alpha antibody|Hypoxia inducible factor 1 alpha isoform I.3 antibody|Hypoxia inducible factor 1 alpha subunit antibody|Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody|Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor) antibody|Hypoxia inducible factor1alpha antibody|Hypoxia-inducible factor 1-alpha antibody|Member of PAS protein 1 antibody|Member of PAS superfamily 1 antibody|Member of the PAS Superfamily 1 antibody|MOP 1 antibody|MOP1 antibody|PAS domain-containing protein 8 antibody|PASD 8 antibody|PASD8 antibody

ID gène: 3091

UniProt: Q16665

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, L'effet Warburg