TP53BP1 anticorps (C-Term)

N° du produit ABIN3044038

Détail du produit anti-TP53BP1 anticorps

Antigène: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Épitope: AA 1955-1972, C-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TP53BP1 est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Tumor suppressor p53-binding protein 1(TP53BP1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Séquence: ERIGFKQHPK YKHDYVSH

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Tumor suppressor p53-binding protein 1(TP53BP1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: tumor protein p53 binding protein 1
Protein Name: Tumor suppressor p53-binding protein 1

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1(1955-1972aa ERIGFKQHPKYKHDYVSH), identical to the related mouse sequence.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat, The detection limit for TP53BP1 is approximately 2.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur TP53BP1

Antigène: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Autre désignation: TP53BP1 (TP53BP1 Produits)

Synonymes: anticorps TP53BP1, anticorps MGC160112, anticorps 53BP1, anticorps Tp53bp1, anticorps m53BP1, anticorps p202, anticorps Trp53bp1, anticorps tumor protein p53 binding protein 1, anticorps tumor suppressor p53-binding protein 1, anticorps tumor protein p53 binding protein 1 (predicted), 5 prime, anticorps transformation related protein 53 binding protein 1, anticorps TP53BP1, anticorps tp53bp1, anticorps LOC100550605, anticorps Trp53bp1, anticorps Tp53bp1

Sujet: TP53BP1(Tumor Protein p53-Binding Protein 1), also called 53BP1, is a protein that in humans is encoded by the TP53BP1 gene. Iwabuchi et al.(1998) mapped the TP53BP1 gene to 15q15-q21 by FISH. Iwabuchi et al.(1994) showed that TP53BP1 binds to the conformationally sensitive central domain of wildtype p53 but not to mutant p53 in vitro. Immunoblot analysis by Iwabuchi et al.(1998) showed that expression of TP53BP1 or TP53BP2 enhances the transactivation function of p53 and induces the expression of p21(CDKN1A). Wang et al.(2002) used small interfering RNA directed against TP53BP1 in mammalian cells to demonstrate that TP53BP1 is a key transducer of the DNA damage checkpoint signal. TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation.

Synonyms: 53 BP1 antibody|53BP1 antibody|FLJ41424 antibody|MGC138366 antibody|p202 antibody|p53 binding protein 1 antibody|p53 BP1 antibody|p53-binding protein 1 antibody|p53BP1 antibody|TP53 BP1 antibody|TP53B_HUMAN antibody|TP53BP1 antibody|TRP53 BP1 antibody|Tumor protein 53 binding protein 1 antibody|Tumor protein p53 binding protein 1 antibody|Tumor suppressor p53 binding protein 1 antibody|Tumor suppressor p53-binding protein 1 antibody

UniProt: Q12888

Pathways: Réparation de l'ADN