GDNF anticorps (Glial Cell Line Derived Neurotrophic Factor) (AA 142-158)

Details for Product anti-GDNF Antibody No. ABIN3044406
Antigène
  • gdnf
  • GDNF
  • AI385739
  • gdnfa
  • hfb1-gdnf
  • ATF1
  • ATF2
  • HFB1-GDNF
  • HSCR3
  • glial cell derived neurotrophic factor
  • glial cell derived neurotrophic factor a
  • glial cell line derived neurotrophic factor
  • glial cell derived neurotrophic factor L homeolog
  • Gdnf
  • gdnfa
  • GDNF
  • gdnf.L
Épitope
AA 142-158, Middle Region
15
14
9
9
6
5
3
2
2
1
1
1
1
1
1
1
Reactivité
Humain, Rat (Rattus)
171
87
61
1
Hôte
Lapin
165
10
8
4
2
Clonalité
Polyclonal
Conjugué
Cet anticorp GDNF est non-conjugé
19
9
6
5
5
5
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
161
62
47
32
26
26
13
12
11
6
6
4
3
2
1
1
Options
Fonction Rabbit IgG polyclonal antibody for Glial cell line-derived neurotrophic factor(GDNF) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Immunogène A synthetic peptide corresponding to a sequence in the middle region of human GDNF(142-158aa FRYCSGSCDAAETTYDK), different from the related mouse sequence by three amino acids and rat sequence by two amino acids.
Séquence FRYCSGSCDA AETTYDK
Isotype IgG
Réactivité croisée (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Attributs du produit Rabbit IgG polyclonal antibody for Glial cell line-derived neurotrophic factor(GDNF) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: glial cell derived neurotrophic factor
Protein Name: Glial cell line-derived neurotrophic factor(hGDNF)
Purification Immunogen affinity purified.
Plasmids, Primers & others Plasmids, Primers & others GDNF products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation GDNF (GDNF Antibody Extrait)
Sujet GDNF, Glial cell-derived neurotrophic factor, is a protein that, in humans, is encoded by the GDNF gene. GDNF is a small protein that potently promotes the survival of many types of neurons. GDNF is a founding member of the GDNF family of ligands(GFL). The GDNF gene is mapped to human chromosome 5p13.1-p12 by fluorescence in situ hybridization(FISH). This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosisof motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET(rearranged during transfection) protooncogene.

Synonyms: gdnf antibody|GDNF_HUMAN antibody|Glial cell derived neurotrophic factor antibody|Glial Cell Line Derived Neurotrophic Factor antibody|Glial cell line-derived neurotrophic factor antibody|Glial derived neurotrophic factor antibody|HFB1 GDNF antibody|hGDNF antibody|HSCR3 antibody
UniProt P39905
Pathways Signalisation RTK, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration
Indications d'application WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Commentaires

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Agent conservateur Thimerosal (Merthiolate), Sodium azide
Précaution d'utilisation This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Conseil sur la manipulation Avoid repeated freezing and thawing.
Stock 4 °C/-20 °C
Stockage commentaire At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Date de péremption 12 months
Images (Fournisseur)
Immunohistochemistry (IHC) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 142-158), (Middle Region) antibody (ABIN3044406) Anti-GDNF antibody, IHC(P) IHC(P): Rat Brain Tissue
Western Blotting (WB) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 142-158), (Middle Region) antibody (ABIN3044406) Lane 4: SMMC Cell Lysate
Immunohistochemistry (IHC) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 142-158), (Middle Region) antibody (ABIN3044406) Anti-GDNF antibody, IHC(P) IHC(P): Human Lung Cancer Tissue
Western Blotting (WB) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 142-158), (Middle Region) antibody (ABIN3044406) Anti-GDNF antibody, Western blotting Lane 1: Recombinant Human GDNF Protein 10ng Lane...
Produit citée dans: A, Xu, Zhao, Li, Qi, Li, Wang, Zhou: "Surface functionalization of TiO2 nanotubes with minocycline and its in vitro biological effects on Schwann cells." dans: Biomedical engineering online, Vol. 17, Issue 1, pp. 88, 2018 (PubMed).

Zhang, Shi, Li, Zhang, Hao: "Lipopolysaccharide inhibits the self-renewal of spermatogonial stem cells in vitro via downregulation of GDNF expression in Sertoli cells." dans: Reproductive toxicology (Elmsford, N.Y.), Vol. 45, pp. 87-93, 2014 (PubMed).

Liu, Wang, Liu, Yang, Xu, Ren: "Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction." dans: Neural regeneration research, Vol. 8, Issue 23, pp. 2190-7, 2014 (PubMed).

Li, Xia, Zhang, Wu: "S100B protein, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor in human milk." dans: PLoS ONE, Vol. 6, Issue 6, pp. e21663, 2011 (PubMed).

Avez-vous cherché autre chose?