PIAS1 anticorps (C-Term)

N° du produit ABIN3044474

Détail du produit anti-PIAS1 anticorps

Antigène: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Épitope: AA 636-651, C-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PIAS1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Fonction: Rabbit IgG polyclonal antibody for E3 SUMO-protein ligase PIAS1(PIAS1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Séquence: DTASIFGIIP DIISLD

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for E3 SUMO-protein ligase PIAS1(PIAS1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: protein inhibitor of activated STAT, 1
Protein Name: E3 SUMO-protein ligase PIAS1

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human PIAS1(636-651aa DTASIFGIIPDIISLD), identical to the related mouse and rat sequences.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-PIAS1 (ABIN3044474)

Scortegagna, Berthon, Settas, Giannakou, Garcia, Li, James, Liddington, Vilches-Moure, Stratakis, Ronai: "The E3 ubiquitin ligase Siah1 regulates adrenal gland organization and aldosterone secretion." dans: JCI insight, Vol. 2, Issue 23, (2019) (PubMed).

Détails sur PIAS1

Antigène: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Autre désignation: PIAS1 (PIAS1 Produits)

Synonymes: anticorps 2900068C24Rik, anticorps Ddxbp1, anticorps GBP, anticorps DDXBP1, anticorps GU/RH-II, anticorps ZMIZ3, anticorps ddxbp1, anticorps gbp, anticorps pias, anticorps pias1-A, anticorps xPAIS1, anticorps xPIAS1, anticorps zmiz3, anticorps protein inhibitor of activated STAT 1, anticorps protein inhibitor of activated STAT, 1, anticorps protein inhibitor of activated STAT, 1a, anticorps protein inhibitor of activated STAT 1 L homeolog, anticorps PIAS1, anticorps Pias1, anticorps pias1a, anticorps pias1.L

Sujet: E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. It is mapped to 15q23. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1(signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. On the other hand, PIAS1 also can function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.

Synonyms: DDXBP1 antibody|DEAD/H box binding protein 1 antibody|DEAD/H box-binding protein 1 antibody|E3 SUMO-protein ligase PIAS1 antibody|GBP antibody|Gu binding protein antibody|Gu-binding protein antibody|Pias1 antibody|PIAS1_HUMAN antibody|Protein inhibitor of activated STAT protein 1 antibody|RNA helicase II binding protein antibody|RNA helicase II-binding protein antibody

UniProt: O75925

Pathways: Signalistation JAK/STAT, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C