beta Actin anticorps (Actin, beta) (Cytosolic)

Details for Product anti-ACTB Antibody No. ABIN3044484
Antigène
  • BRWS1
  • PS1TP5BP1
  • ACTA1
  • Actx
  • E430023M04Rik
  • beta-actin
  • ps1tp5bp1
  • act-2
  • actb
  • B-actin
  • LOC100174883
  • LOC100304816
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • Bact
  • ACT-5
  • ACTB
  • B-ACTZF
  • actba
  • bact
  • bactin1
  • bactzf
  • wu:fd18f05
  • Beta-actin
  • actin beta
  • actin, beta
  • cytoplasmic actin OlCA1
  • beta-actin
  • Actin 5C
  • actin gamma 1
  • actin, beta 1
  • actin, beta L homeolog
  • beta actin
  • actin, cytoplasmic 1
  • ACTB
  • Actb
  • actb
  • LOC100174883
  • LOC100304816
  • Act5C
  • ACTG1
  • actb1
  • actb.L
  • LOC100136352
  • LOC109073280
Épitope
Cytosolic, N-Term
130
38
17
17
10
7
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
553
325
280
97
86
74
64
54
51
43
36
33
21
14
14
11
10
10
9
7
6
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Hôte
Souris
355
237
14
7
2
1
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp beta Actin est non-conjugé
34
31
16
7
7
7
7
7
7
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
522
230
158
106
81
68
41
37
34
13
13
12
12
8
2
1
1
1
1
Options
Fonction Mouse IgG monoclonal antibody for beta-Actin, actin, beta (ACTB) detection. Tested with WB, IHC-P in Human,mouse,rat. No cross reactivity with other proteins.
Immunogène slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Séquence Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys
Clone AC-15
Isotype IgG1
Specificité Actin, cytoplasmic 1
Réactivité croisée (Details) No cross reactivity with other proteins.
Attributs du produit Mouse IgG monoclonal antibody for beta-Actin, actin, beta (ACTB) detection. Tested with WB, IHC-P in Human,mouse,rat.
Gene Name: actin, beta
Protein Name: Actin, cytoplasmic 1
Purification Ascites
Plasmids, Primers & others Plasmids, Primers & others beta Actin products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation ACTB (ACTB Antibody Extrait)
Sujet The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

Synonyms: BRWS1|PS1TP5BP1|BETA-ACTIN|ACTIN, CYTOPLASMIC, 1
ID gène 60, 11461, 81822
UniProt P60709, P60710, P60711
Pathways Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
Indications d'application WB: Concentration: 0.25-0.5 μg/mLμg/mL, Species: Human, mouse, rat
IHC-P: Concentration: 0.5-1 μg/mL, Species: Human, mouse, rat
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Commentaires

Antibody can be supported by chemiluminescence kit ABIN921123 in WB, supported by ABIN921230 in IHC(P).

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 1 mL of PBS buffer will yield a concentration of 100 μg/mL.
Concentration 100 μg/mL
Buffer Mouse ascites fluid, 1.2 % sodium acetate, 2 mg BSA, with 0.01 mg Sodium azide as preservative.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid repeated freezing and thawing.
Stock 4 °C/-20 °C
Stockage commentaire At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Date de péremption 12 months
Images (Fournisseur)
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), IHC(P) IHC(P): Human Intestinal Cancer Tissue
Western Blotting (WB) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), Western blotting Lane 1: Rat Ovary Tissue Lysa...
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), IHC(P) IHC(P): Human Lung Cancer Tissue
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), IHC(P) IHC(P): Mouse Intestine Tissue
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), IHC(P) IHC(P): Human Mammary Cancer Tissue
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), IHC(P) IHC(P): Rat Intestine Tissue
Western Blotting (WB) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), Western blotting Lane 1: Rat Liver Tissue Lysa...
Western Blotting (WB) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), Western blotting Lane 1: Rat Ovary Tissue Lysa...
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Zhang, Wu, Zhang, Shang, Fang, Zhuang: "Fibrotic microenvironment promotes the metastatic seeding of tumor cells via activating the fibronectin 1/secreted phosphoprotein 1-integrin signaling." dans: Oncotarget, Vol. 7, Issue 29, pp. 45702-45714, 2018 (PubMed).

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Wang, Lu, You, Huang, Chen, Liu: "The mTOR/AP-1/VEGF signaling pathway regulates vascular endothelial cell growth." dans: Oncotarget, Vol. 7, Issue 33, pp. 53269-53276, 2018 (PubMed).

Wang, Li, Wang, Wang, Yan, Zhang, Ma, Du, Liu, Yang: "MicroRNA-552 enhances metastatic capacity of colorectal cancer cells by targeting a disintegrin and metalloprotease 28." dans: Oncotarget, Vol. 7, Issue 43, pp. 70194-70210, 2018 (PubMed).

Zha, Xu, Chen, Yang, Zhang, Lu, Yu, Li, Wu, Zheng, Li: "Generation of V α13/β21+T cell specific target CML cells by TCR gene transfer." dans: Oncotarget, Vol. 7, Issue 51, pp. 84246-84257, 2018 (PubMed).

Zhang, Liu, Lian, Liao, Chen, Qin, Wang, Jiang, Wang, Gong: "Monoacylglycerol Lipase: A Novel Potential Therapeutic Target and Prognostic Indicator for Hepatocellular Carcinoma." dans: Scientific reports, Vol. 6, pp. 35784, 2018 (PubMed).

Liu, Gao, Wang, Ding, Lv, Gao, Wang, Niu: "Ubenimex attenuates acquired sorafenib resistance in renal cell carcinoma by inhibiting Akt signaling in a lipophagy associated mechanism." dans: Oncotarget, Vol. 7, Issue 48, pp. 79141-79153, 2018 (PubMed).

Xiao, Zhang, Li, Wu, Liu, Tan, Du: "Dioscin augments HSV-tk-mediated suicide gene therapy for melanoma by promoting connexin-based intercellular communication." dans: Oncotarget, Vol. 8, Issue 1, pp. 798-807, 2018 (PubMed).

Ouyang, Chang, Fang, Qin, Qu, Cheng: "Estrogen-induced SDF-1α production promotes the progression of ER-negative breast cancer via the accumulation of MDSCs in the tumor microenvironment." dans: Scientific reports, Vol. 6, pp. 39541, 2018 (PubMed).

Zhao, Xu, Xu, Qi, Li, Cheng, Liu, Wu, Gao, Ye: "The overexpressed functional transient receptor potential channel TRPM2 in oral squamous cell carcinoma." dans: Scientific reports, Vol. 6, pp. 38471, 2018 (PubMed).

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Li, Fang, Jiang, Wang, Ma, Kang, Zhang, Miao, Wang, Guo, Zhang, Guo, Zhao, Yang, Tian, Xiao: "STAT3 and NF-κB are Simultaneously Suppressed in Dendritic Cells in Lung Cancer." dans: Scientific reports, Vol. 7, pp. 45395, 2018 (PubMed).

Duan, Guo, Li, Yuan: "Antifatigue Effect of Luteolin-6-C-Neohesperidoside on Oxidative Stress Injury Induced by Forced Swimming of Rats through Modulation of Nrf2/ARE Signaling Pathways." dans: Oxidative medicine and cellular longevity, Vol. 2017, pp. 3159358, 2018 (PubMed).

Xie, Yu, Chen, Zhang, Ma, Di: "HMGB1 regulates P-glycoprotein expression in status epilepticus rat brains via the RAGE/NF-κB signaling pathway." dans: Molecular medicine reports, Vol. 16, Issue 2, pp. 1691-1700, 2018 (PubMed).

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