PPP2CA anticorps (N-Term)

N° du produit ABIN3044492

Détail du produit anti-PPP2CA anticorps

Antigène: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Épitope: AA 6-20, N-Term

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PPP2CA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PPP2CA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Séquence: FTKELDQWIE QLNEC

Réactivité croisée (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Attributs du produit: Rabbit IgG polyclonal antibody for Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PPP2CA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: protein phosphatase 2, catalytic subunit, alpha isozyme
Protein Name: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PP2A-alpha)

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human PP2A-alpha (6-20aa FTKELDQWIEQLNEC), identical to the related rat and mouse sequences.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-PPP2CA (ABIN3044492)

Tian, Qiu, Zhao, Li, Guo: "Sphingomyelinase-induced ceramide production stimulate calcium-independent JNK and PP2A activation following cerebral ischemia." dans: Brain injury, Vol. 23, Issue 13-14, pp. 1073-80, (2009) (PubMed).

Détails sur PPP2CA

Antigène: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Autre désignation: PPP2CA (PPP2CA Produits)

Synonymes: anticorps PP2Ac, anticorps PP2CA, anticorps PP2Calpha, anticorps RP-C, anticorps PP2A, anticorps R75353, anticorps Pp2a1, anticorps pp2ac, anticorps zgc:110641, anticorps protein phosphatase 2 catalytic subunit alpha, anticorps protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform, anticorps protein phosphatase 2, catalytic subunit, alpha isozyme, anticorps protein phosphatase 2 catalytic subunit alpha L homeolog, anticorps protein phosphatase 2, catalytic subunit, beta isozyme, anticorps serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, anticorps PPP2CA, anticorps Ppp2ca, anticorps ppp2ca.L, anticorps ppp2cb, anticorps LOC471633, anticorps ppp2ca

Sujet: The catalytic subunit of human protein phosphatase 2A(PPP2CA) encodes a 309-amino acid polypeptide.It is localized to chromosome 5. The gene(approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue(Leu309) of protein serine/threonine phosphatase 2A catalytic subunit(PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.

Synonyms: PP2A alpha antibody|PP2A-alpha antibody|PP2AA_HUMAN antibody|PP2Aalpha antibody|PP2Ac antibody|PP2CA antibody|PP2Calpha antibody|PPP2CA antibody|Protein phosphatase 2(formerly 2A) catalytic subunit alpha isoform antibody|Protein phosphatase 2 catalytic subunit alpha isoform antibody|Protein phosphatase 2, catalytic subunit, alpha isozyme antibody|Protein phosphatase 2A catalytic subunit, alpha isoform antibody|Replication protein C antibody|RP C antibody|RP-C antibody|RPC antibody|Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody|Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody

UniProt: P67775

Pathways: Signalisation PI3K-Akt, Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades