ABCB11 anticorps (AA 705-718)

N° du produit ABIN305775

Cet anticorps anti-ABCB11 est un anticorps Souris Monoclonal détectant ABCB11 dans WB, IHC, ICC et IHC (p). Adapté pour Humain.

Aperçu rapide pour ABCB11 anticorps (AA 705-718) (ABIN305775)

Antigène: ABCB11 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 11 (ABCB11))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ABCB11 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ABCB11 anticorps

Épitope: AA 705-718

Marque: IHC-plus™

Specificité: Recognizes human BSEP. Does not cross-react with human P-glycoprotein.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda (100%) Marmoset, Dog (93%).

Purification: Protein G purified

Immunogène: Synthetic peptide corresponding to aa 705-718 (Y705LVHEPPLAVVDHK718) of human BSEP (bile salt export pump). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda (100%), Marmoset, Dog (93%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Information d'application

Indications d'application: Approved: ICC, IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.Western Blot: 1:50-1:200Immunocytochemistry: Cytospin preparationsOptimal dilutions to be determined by the researcher.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS  pH 7.2, 0.7 % BSA, 100nM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur ABCB11

Antigène: ABCB11 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 11 (ABCB11))

Autre désignation: ABCB11 / BSEP

Sujet: Name/Gene ID: ABCB11
Subfamily: ATP-binding cassette - ABCB/MDR
Family: Transporter

Synonyms: ABCB11, ABC16, Bile salt export pump, BSEP, PGY4, Sister p-glycoprotein, BRIC2, PFIC-2, PFIC2, SPGP

ID gène: 8647

UniProt: O95342