This antibody specifically recognises an extracellular conformational epitope of CD243, also known as MDR1 (multidrug resistance protein 1) and Pgp (Pglycoprotein), a transmembrane protein and member of the ABC transporter (ATPbinding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds. Clone UIC2 can be used in a shift assay to selectively demonstrate the expression and functional activity of CD243 in a target cell. Clone UIC2 does not cross-react with mitochondrial pyruvate carboxylase. We recommend the use of AM05632LE-N for Functional Studies.
Aucune reactivité croisée
Souris, Rat (Rattus)
Réactivité croisée (Details)
Species reactivity (tested):Human, Primate.
Attributs du produit
Synonyms: Multidrug resistance protein 1, P-glycoprotein 1, ABCB1, PGY1, ATP-binding cassettesub-family B member 1
Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogène
Mouse Balb/c 3T3 fibroblasts transfected with human CD243 cDNA.
Immunoprecipitation. Immunofluorescence. Flow Cytometry: Use 10 μL of neat MDR1/CD243 antibody to label 1x10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/200, This product may benefit from antigenretrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled bytrained staff only.
Date de péremption
12 months
Antigène
ABCB1
(ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))
anticorps ABC20, anticorps CD243, anticorps CLCS, anticorps GP170, anticorps MDR1, anticorps P-GP, anticorps PGY1, anticorps Abcb1, anticorps Mdr1a, anticorps p-gp, anticorps xemdr, anticorps Mdr1, anticorps Mdr1b, anticorps Pgy-1, anticorps Pgy1, anticorps mdr, anticorps ABCB1, anticorps PGP1, anticorps ATP binding cassette subfamily B member 1, anticorps ATP binding cassette subfamily B member 1A, anticorps ATP binding cassette subfamily B member 1 L homeolog, anticorps ATP-binding cassette, sub-family B (MDR/TAP), member 1B, anticorps ABCB1, anticorps Abcb1a, anticorps abcb1.L, anticorps Abcb1b, anticorps Abcb1
Sujet
CD243 is also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein), a transmembrane protein and member of the ABC transporter (ATP-binding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds. CD243 is expressed at low levels in the cell membrane of peripheral blood leucocytes, and constitutively expressed on the apical plasma membrane of excretory epithelial cells of the kidney, liver, brain and small intestine. CD243 mediates resistance to many chemotherapeutic agents used for tumour suppression and is therefore of special interest to oncologists. Clone UIC2 is a strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs.Synonyms: ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1, PGY1