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HBEGF anticorps (Bound, EGF Like Domain, Soluble)

HBEGF Reactivité: Humain WB, ELISA, IP, ICC, IF Hôte: Souris Monoclonal 4G10 unconjugated
N° du produit ABIN3201018
  • Antigène Voir toutes HBEGF Anticorps
    HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
    Épitope
    • 15
    • 15
    • 14
    • 9
    • 7
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Bound, EGF Like Domain, Soluble
    Reactivité
    • 77
    • 20
    • 19
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 71
    • 9
    Souris
    Clonalité
    • 68
    • 11
    • 1
    Monoclonal
    Conjugué
    • 27
    • 10
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Cet anticorp HBEGF est non-conjugé
    Application
    • 36
    • 29
    • 26
    • 15
    • 13
    • 10
    • 7
    • 7
    • 6
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Réactivité croisée (Details)
    Does not react with mouse.
    Stérilité
    Sterile filtered
    Immunogène
    RecombinanthumanHB-EGFectodomainexpressed inSF21 cell
    Clone
    4G10
    Isotype
    IgG1
  • Indications d'application
    The antibody can react with both soluble and cell-surface attached forms of HG-EGF.
    1) Westernblotting: 0. 2~1 g/mL
    2) Immunoprecipitation: 1 g/mL
    3) Indirect immuno-fluorescence staining: 5~11 g/mL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS with 50 % Glycerol
    Agent conservateur
    Azide free
    Stock
    -20 °C
    Stockage commentaire
    Upon arrival centrifuge briefly, aliquote and store at -20 C.
  • Miyamoto, Hirata, Yamazaki, Kageyama, Hasuwa, Mizushima, Tanaka, Yagi, Sonoda, Kai, Kanoh, Nakano, Mekada: "Heparin-binding EGF-like growth factor is a promising target for ovarian cancer therapy." dans: Cancer research, Vol. 64, Issue 16, pp. 5720-7, (2004) (PubMed).

    Prenzel, Zwick, Daub, Leserer, Abraham, Wallasch, Ullrich: "EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF." dans: Nature, Vol. 402, Issue 6764, pp. 884-8, (2000) (PubMed).

    Iwamoto, Higashiyama, Mitamura, Taniguchi, Klagsbrun, Mekada et al.: "Heparin-binding EGF-like growth factor, which acts as the diphtheria toxin receptor, forms a complex with membrane protein DRAP27/CD9, which up-regulates functional receptors and diphtheria toxin ..." dans: The EMBO journal, Vol. 13, Issue 10, pp. 2322-30, (1994) (PubMed).

    Higashiyama, Abraham, Miller, Fiddes, Klagsbrun: "A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF." dans: Science (New York, N.Y.), Vol. 251, Issue 4996, pp. 936-9, (1991) (PubMed).

  • Antigène
    HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
    Autre désignation
    HB-EGF (HBEGF Produits)
    Synonymes
    anticorps hbegf, anticorps zgc:165443, anticorps HBEGF, anticorps zgc:175091, anticorps DTR, anticorps DTS, anticorps DTSF, anticorps HEGFL, anticorps Dtr, anticorps GFHB, anticorps Hb-egf, anticorps Hegfl, anticorps AW047313, anticorps Dts, anticorps HB-EGF, anticorps heparin-binding EGF-like growth factor b, anticorps heparin binding EGF like growth factor, anticorps Heparin-binding EGF-like growth factor, anticorps heparin-binding EGF-like growth factor a, anticorps heparin-binding EGF-like growth factor, anticorps hbegfb, anticorps HBEGF, anticorps hbegf, anticorps hbegfa, anticorps Hbegf
    Sujet
    Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is synthesized as a membrane-anchored precursor that is proteolytically cleaved to release the soluble mature growth factor, HB-EGF. The former functions as juxtacrine and the latter as paracrine growth factor. Soluble HB-EGF shows several forms in Western blotting with apparent molecular weights 19~27 kDa due to heterogeneous O-glycosylation and N-terminal truncation. HB-EGF activates EGFR and ErbB4 and promotes the development in many tissues. In human ProHB-EGF is the cellular receptor for diphtheria toxin. Non-toxic mutant of diphtheria toxin, CRM197, inhibits HB-EGF function. As HB-EGF level is elevated in most ovarian cancer, CRM197 is being tested as an anticancer drug. The hybridoma clone 4G10 was established and characterized in the laboratoryof Prof. E.Mekadaof OsakaUniversity, who is a leadingscientist inthis field.
    UniProt
    Q99075
    Pathways
    Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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