TOP2 anticorps (C-Term, N-Term)

N° du produit ABIN334538

Détail du produit anti-TOP2 anticorps

Antigène: TOP2 (DNA Topoisomerase II (TOP2))

Épitope: C-Term, N-Term

Reactivité: Arabidopsis thaliana, Vicia faba

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TOP2 est non-conjugé

Application: Western Blotting (WB), Immunolocalization (IL)

Specificité: Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis cells the antibody detects a 170 kDa protein exclusively in the nulear fraction.

Réactivité croisée (Details): Not reactive in: Nicotiana tabacum

Homologie: Brassica rapa, Chlamydomonas reinhardtii, Chlorella vulgaris, Citrus clementina, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Ostreococcus tauri, Panicum italicum, Phaseolus vulgaris, Physcomitrella patens, Pinus sitchensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Vitis vinifera, Volvox caterii

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 164 / 170 kDa (Arabidopsis thaliana)

Purification: serum

Immunogène: The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890, protein accesion number P30182) with an N-terminal hexahistidine tag was expressed in E.coli and purified by Ni2+ affinity chromatography.

Information d'application

Indications d'application: 1: 2000 (WB), 1: 500 (IL)

Commentaires:

Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Références pour anticorps anti-TOP2 (ABIN334538)

Xie, Lam: "Characterization of a DNA Topoisomerase II cDNA from Arabidopsis thaliana." dans: Plant physiology, Vol. 106, Issue 4, pp. 1701-2, (1995) (PubMed).

Détails sur TOP2

Antigène: TOP2 (DNA Topoisomerase II (TOP2))

Autre désignation: TOP2 (TOP2 Produits)

Sujet: AGI Code: At3g23890
Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2, TOPOISOMERASE II

Poids moléculaire: expected: 164 kDa, apparent: 170 kDa (Arabidopsis thaliana)

UniProt: P30182

Pathways: Mitotic G1-G1/S Phases