RFP anticorps (Red Fluorescent Protein)

Details for Product anti-RFP Antibody No. ABIN334653
Antigène
  • RFP
  • RNF76
  • tripartite motif containing 27
  • TRIM27
Reactivité
Discosoma
91
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8
5
1
1
Hôte
Rat
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14
6
1
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp RFP est non-conjugé
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4
4
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
81
45
28
22
18
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6
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Options
Fonction For biochemical analysis of RFP-tagged fusion proteins.
Clone 5F8
Isotype IgG2a
Specificité 5F8 efficiently recognizes monomeric red fluorescent proteins from Discosoma sp. derived from DsRed such as mRFP, mCherry or mPlum but no green fluorescent proteins.
Tested on mRFP, mCherry, mPlum, mOrange, mRFPRuby, tdTomato, DsRed
Sensibilité Sensitivity range of rat RFP antibody 5F8 goes down to 1.25 ng of purified RFP protein.
Attributs du produit Use this rat monoclonal antibody (MAb) against monomeric RFPs, designated as RFP antibody [5F8]. The 5F8 MAb is characterized by high affinity and specificity against DsRed derivatives. It is suitable for ELISA, immunoblotting, immunoprecipitation, and immunofluorescence assays.
Purification Hybridoma supernatant. The Hybridoma was cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kDa exclusion membrane to an antibody concentration of 1 mg/ml.
Plasmids, Primers & others Plasmids, Primers & others RFP products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation RFP (RFP Antibody Extrait)
Sujet Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells. In addition to the prominent green fluorescent protein (GFP) from Aequorea victoria, a red fluorescent protein (DsRed) was discovered in the coral Discosoma sp. Mutagenesis of the red progenitor have resulted in several monomeric red FPs (mRFP1, mCherry, mOrange, mPlum, etc.) which became attractive tools for a number of applications.
Indications d'application Western blot: recommended starting concentration 1/1000
IF: recommended starting concentration 1/1000
IP: recommended starting volume 10 µL/IP
Restrictions For Research Use only
Format Liquid
Concentration 1mg/mL
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Do not freeze.
Stock 4 °C
Date de péremption 12 months
Images (Fournisseur)
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) antibody (ABIN334653) primary antibody: 0.2 ug of multi-red antibody 5F8 incubation for 1 h at RT secondary...
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) antibody (ABIN334653) RFP antibody 5F8 shows no crossreactivity to any cellular protein. Detects a band of ...
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) antibody (ABIN334653) Sensitivity range of rat RFP antibody 5F8 goes down to1.25 ng of purified RFP protein.
Immunoprecipitation (IP) image for anti-Red Fluorescent Protein (RFP) antibody (ABIN334653) IP, Co-IP: For Co-immunoprecipitation, 4 µg of RFP antibody 5F8 was used incubation f...
Immunofluorescence (IF) image for anti-Red Fluorescent Protein (RFP) antibody (ABIN334653) Immunofluorescence: HeLa cells stably expressing mCherry-PCNA, 3.7% PFA fixed. Primar...
Produit citée dans: Bieluszewska, Weglewska, Bieluszewski, Lesniewicz, Poreba: "PKA-binding domain of AKAP8 is essential for direct interaction with DPY30 protein." dans: The FEBS journal, Vol. 285, Issue 5, pp. 947-964, 2019 (PubMed).

Morland, Andersson, Haugen, Hadzic, Kleppa, Gille, Rinholm, Palibrk, Diget, Kennedy, Stølen, Hennestad, Moldestad, Cai, Puchades, Offermanns, Vervaeke, Bjørås, Wisløff, Storm-Mathisen, Bergersen: "Exercise induces cerebral VEGF and angiogenesis via the lactate receptor HCAR1." dans: Nature communications, Vol. 8, pp. 15557, 2018 (PubMed).

Huang, Gu, Zhang, Yan, Kong, Kong, Guo, Qiu, Wang, Jing, Xing, Ye, Wu, Zhang, Zheng, Gijzen, Wang, Dong: "An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity." dans: Nature communications, Vol. 8, Issue 1, pp. 2051, 2018 (PubMed).

Früholz, Fäßler, Kolukisaoglu, Pimpl: "Nanobody-triggered lockdown of VSRs reveals ligand reloading in the Golgi." dans: Nature communications, Vol. 9, Issue 1, pp. 643, 2018 (PubMed).

Moreno-Juan, Filipchuk, Antón-Bolaños, Mezzera, Gezelius, Andrés, Rodríguez-Malmierca, Susín, Schaad, Iwasato, Schüle, Rutlin, Nelson, Ducret, Valdeolmillos, Rijli, López-Bendito: "Prenatal thalamic waves regulate cortical area size prior to sensory processing." dans: Nature communications, Vol. 8, pp. 14172, 2018 (PubMed).

Ragni, Diguet, Le Garrec, Novotova, Resende, Pop, Charon, Guillemot, Kitasato, Badouel, Dufour, Olivo-Marin, Trouvé, McNeill, Meilhac: "Amotl1 mediates sequestration of the Hippo effector Yap1 downstream of Fat4 to restrict heart growth." dans: Nature communications, Vol. 8, pp. 14582, 2018 (PubMed).

Ikeda, Hikichi, Miura, Shibata, Mitsunaga, Yamada, Woltjen, Miyamoto, Hiratani, Yamada, Hotta, Yamamoto, Okita, Masui: "Srf destabilizes cellular identity by suppressing cell-type-specific gene expression programs." dans: Nature communications, Vol. 9, Issue 1, pp. 1387, 2018 (PubMed).

Huang, Niesman, Arasu, Lee, De La Cruz, Callejas, Hong, Lois: "Tracing neuronal circuits in transgenic animals by transneuronal control of transcription (TRACT)." dans: eLife, Vol. 6, 2018 (PubMed).

Quattrocolo, Fishell, Petros: "Heterotopic Transplantations Reveal Environmental Influences on Interneuron Diversity and Maturation." dans: Cell reports, Vol. 21, Issue 3, pp. 721-731, 2018 (PubMed).

Froux, Le Bon-Jego, Miguelez, Normand, Morin, Fioramonti, Barresi, Frick, Baufreton, Taupignon: "D5 dopamine receptors control glutamatergic AMPA transmission between the motor cortex and subthalamic nucleus." dans: Scientific reports, Vol. 8, Issue 1, pp. 8858, 2018 (PubMed).

Andersen, Tampakakis, Jimenez, Kannan, Miyamoto, Shin, Saberi, Murphy, Sulistio, Chelko, Kwon: "Precardiac organoids form two heart fields via Bmp/Wnt signaling." dans: Nature communications, Vol. 9, Issue 1, pp. 3140, 2018 (PubMed).

Mahuzier, Shihavuddin, Fournier, Lansade, Faucourt, Menezes, Meunier, Garfa-Traoré, Carlier, Voituriez, Genovesio, Spassky, Delgehyr: "Ependymal cilia beating induces an actin network to protect centrioles against shear stress." dans: Nature communications, Vol. 9, Issue 1, pp. 2279, 2018 (PubMed).

Collins, Malacrida, Burke, Kiely, Dunleavy: "ATP synthase F1 subunits recruited to centromeres by CENP-A are required for male meiosis." dans: Nature communications, Vol. 9, Issue 1, pp. 2702, 2018 (PubMed).

Dreissig, Schiml, Schindele, Weiss, Rutten, Schubert, Gladilin, Mette, Puchta, Houben: "Live-cell CRISPR imaging in plants reveals dynamic telomere movements." dans: The Plant journal : for cell and molecular biology, Vol. 91, Issue 4, pp. 565-573, 2018 (PubMed).

Bataillé, Boukhatmi, Frendo, Vincent: "Dynamics of transcriptional (re)-programming of syncytial nuclei in developing muscles." dans: BMC biology, Vol. 15, Issue 1, pp. 48, 2018 (PubMed).

Tan, Liu, Xi, Lou, Zhu, Guo, Mei, Duan: "Glia-derived ATP inversely regulates excitability of pyramidal and CCK-positive neurons." dans: Nature communications, Vol. 8, pp. 13772, 2018 (PubMed).

Han, Xiong, Xu, Tian, Wang: "The β-alanine transporter BalaT is required for visual neurotransmission in Drosophila." dans: eLife, Vol. 6, 2018 (PubMed).

Wu, Capelli, Bouvier, Goulding, Arber, Fortin: "A V0 core neuronal circuit for inspiration." dans: Nature communications, Vol. 8, Issue 1, pp. 544, 2018 (PubMed).

Lampada, OPrey, Szabadkai, Ryan, Hochhauser, Salomoni: "mTORC1-independent autophagy regulates receptor tyrosine kinase phosphorylation in colorectal cancer cells via an mTORC2-mediated mechanism." dans: Cell death and differentiation, Vol. 24, Issue 6, pp. 1045-1062, 2018 (PubMed).

Chen, Zhang, Liu, Adams, Eilken, Stehling, Corada, Dejana, Zhou, Adams: "Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells." dans: Nature communications, Vol. 7, pp. 12422, 2018 (PubMed).

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