KIAA0319 anticorps (AA 391-403)

N° du produit ABIN342965

Détail du produit anti-KIAA0319 anticorps

Antigène: KIAA0319

Épitope: AA 391-403

Reactivité: Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp KIAA0319 est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Mouse KIAA0319. The variants XP_341528.2 and XP_001070349.1 represent identical protein. This antibody is expected to cross-react with mouse KIAA0319 isoform 1 (NP_001074520.1), isoform 2 (XP_994023.1) and isoform 4 (XP_913491.1).

Homologie: Percent identity by BLAST analysis: Mouse, Rat (100%) Hamster (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-QGKIKQENKPTLH from an internal region of mouse KIAA0319 (NP_001074520.1). Percent identity by BLAST analysis: Mouse, Rat (100%), Hamster (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: Peptide-ELISA (1:128000, 0.5 - 1.5 μg/mL), WB (0.5 - 1.5 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 150kD band observed in Mouse Brain lysates (calculated MW of 118kD according to Mouse NP_001074520.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Velayos-Baeza, Hum Mol Genet. 2008 Mar 15,17(6):859-71, PMID: 18063668). Recommended concentration: 0.5-1.5 μg/mL. An additional band of unknown identity was also consistently observed at 25kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur KIAA0319

Antigène: KIAA0319

Autre désignation: KIAA0319 (KIAA0319 Produits)

Synonymes: anticorps DYLX2, anticorps DYX2, anticorps 4930451E12Rik, anticorps Kiaa0319, anticorps KIAA0319, anticorps RIKEN cDNA D130043K22 gene, anticorps KIAA0319 ortholog, anticorps similar to mKIAA0319 protein, anticorps KIAA0319, anticorps D130043K22Rik, anticorps RGD1307443

Sujet: Name/Gene ID: KIAA0319

Synonyms: KIAA0319, Dyslexia susceptibility 2, DYX2, DYLX2

ID gène: 9856

NCBI Accession: NP_001074520

UniProt: Q5VV43