Vimentin anticorps
Aperçu rapide pour Vimentin anticorps (ABIN356450)
Antigène
Voir toutes Vimentin (VIM) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
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This antibody reacts with Human Vimentin. Other species not tested.
Cellular Localization: Cytoplasmic. -
Purification
- Protein A Chromatography.
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Immunogène
- Purified human Vimentin.
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Isotype
- IgG
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Indications d'application
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Immunohistochemistry: 1/50-1/100 using polymer system or streptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears do not requireantigen retrieval. Positive control: Sarcomas, melanomas, Hela cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 3.0 mg/mL
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Buffer
- 10 mM PO4, 200 mM NaCl, pH 7.4, 0.05 % Sodium Azide, 1 % BSA
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Vimentin (VIM)
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Autre désignation
- Vimentin
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Sujet
- Vimentin is 57 kDa intermediate filament proteins in mesenchymal cells. It is attached to the nucleus, endoplasmic reticulum and mitochondria either laterally or terminally. Vimentin is important in the differential diagnosis of undifferentiated neoplasm.Synonyms: VIM
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ID gène
- 7431
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NCBI Accession
- NP_003371
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UniProt
- P08670
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Pathways
- Caspase Cascade in Apoptosis
Antigène
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