Protein A column, followed by two-step phosphospecific peptide affinity purification
Immunogène
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S254 of human ENT1.
SLC29A1
Reactivité: Humain
WB, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: 1/1,000. Dot Blot: 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Dephoure, Zhou, Villén, Beausoleil, Bakalarski, Elledge, Gygi: "A quantitative atlas of mitotic phosphorylation." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, (2008) (PubMed).
Antigène
SLC29A1
(Solute Carrier Family 29 (Nucleoside Transporters), Member 1 (SLC29A1))
anticorps ENT1, anticorps rENT1, anticorps 1200014D21Rik, anticorps AA407560, anticorps solute carrier family 29 member 1 (Augustine blood group), anticorps solute carrier family 29 member 1, anticorps solute carrier family 29 (nucleoside transporters), member 1, anticorps SLC29A1, anticorps Slc29a1
Sujet
ENT1 is a member of the equilibrative nucleoside transporter family. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies.Synonyms: Equilibrative nucleoside transporter 1, Solute carrier family 29 member 1