ATP6V0D2 anticorps (Isoform 1)

N° du produit ABIN361273

Détail du produit anti-ATP6V0D2 anticorps

Antigène: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Épitope: Isoform 1

Reactivité: Tomato, Arabidopsis thaliana, Nicotiana tabacum

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ATP6V0D2 est non-conjugé

Application: Western Blotting (WB)

Réactivité croisée (Details): Specificity has been determined using ELISA competition experiments using conjugate of 5-Methoxytryptamine-Glutaric anhydride Compounds Cross-reactivity ratio (a) 5-Methoxytryptamine-S-BSA: 1, Free melatonin 1/ 130, 5-Hydroxytryptamine-A-BSA 1/ 50 000, DL 5-Methoxytryptophan-SA-BSA 1/>50000, Tryptamine-SA-BSA 1/>20 000 (a) : 5-Methoxytryptamine-GA-BSA concentration/ conjugated close structure concentration at half displacement.

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 26 / 31 kDa (Arabidopsis thaliana)

Purification: serum

Immunogène: KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).

Information d'application

Indications d'application: Recommended Dilution: 1 : 1 000 with alkaline phosphatase or 1 : 3000 with ECL (WB).

Commentaires:

V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 300 µL of sterile water.

Conseil sur la manipulation: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Stock: -20 °C

Détails sur ATP6V0D2

Antigène: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Autre désignation: V-ATPase (ATP6V0D2 Produits)

Synonymes: anticorps ATP6D2, anticorps VMA6, anticorps AI324824, anticorps V-ATPase, anticorps 1620401A02Rik, anticorps ATPase H+ transporting V0 subunit d2, anticorps ATPase, H+ transporting, lysosomal V0 subunit D2, anticorps ATPase H+ transporting V0 subunit D2, anticorps ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2 L homeolog, anticorps ATP6V0D2, anticorps Atp6v0d2, anticorps atp6v0d2.L

Sujet: AGI Code: At4g11150
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

Poids moléculaire: expected: 26 kDa, apparent: 31 kDa (Arabidopsis thaliana)

UniProt: Q39258

Pathways: Transition Metal Ion Homeostasis, Proton Transport