tyrosine Hydroxylase (TH) (pSer40) anticorps

Détails pour le produit réf. ABIN361503, Fournisseur: Connectez-vous pour afficher
Antigène
  • DYT14
  • DYT5b
  • TYH
  • The
  • CG10118
  • DH65B
  • DTH
  • Dmel\\CG10118
  • Pale
  • Ple
  • TH
  • Th
  • VII
  • dTH1
  • dTH65B
  • pale/ple
  • th
  • dyt14
  • dyt5b
  • tyh
  • tyrosine hydroxylase
  • pale
  • tyrosine 3-monooxygenase
  • tyrosine hydroxylase S homeolog
  • TH
  • Th
  • ple
  • th
  • th.S
Épitope
pSer40
41
28
25
24
17
16
6
5
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Rat (Rattus)
234
211
152
20
19
18
11
11
5
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Hôte
Lapin
233
51
16
15
10
Clonalité
Polyclonal
Conjugué
Inconjugué
5
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
278
189
146
67
54
40
40
28
18
15
13
7
4
3
2
1
Options
Fournisseur
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N° du produit (Fournisseur)
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Immunogène Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser40 conjugated to KLH
Specificité Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectiity over dephospho TH.
 Réactivité croisée Mammifères
Purification Antigen Affinity Purified from Pooled Serum
Antigène
Autre désignation Tyrosine Hydroxylase (TH Antibody Extrait)
Sujet Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson?s disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000, Salvatore et al., 2001, Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982, Haycock et al., 1992, Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs. Anti-Phospho Ser40 Tyrosine Hydroxylase Western blot of recombinant phospho- and dephospho-TH showing selective immunolabeling by the phospho-specific antibody of the ~60k TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and dephospho-TH, while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH. Immunohistochemical staining of retina with the pan-tyrosine hydroxylase (pan-TH) and phospho-specific tyrosine hydroxylase (phospho-TH) antibodies. The pan-TH antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the phospho-TH antibody selectively labels only the two amacrine cells in this light-stimulated retina example.
Poids moléculaire '60 kDa
ID gène 25085
UniProt P04177
Pathways Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour
Indications d'application Recommended Dilution: WB: 1:1000 IF (frozen sections, Witkovsky et al., 2000): 1:1000 IHC (frozen sections, Witkovsky et al., 2000): 1:1000 Quality Control: Western blots performed on each lot.
Restrictions For Research Use only
Format Liquid
Buffer 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
Stock -20 °C
Images (Fournisseur)
Western Blotting (WB) image for anti-tyrosine Hydroxylase (TH) (pSer40) antibody (ABIN361503) Western blots of recombinant phospho- and dephospho-TH showing selective immunolabeli...
 image for anti-tyrosine Hydroxylase (TH) (pSer40) antibody (ABIN361503) Immunohistochemical staining of retina with the pan-tyrosine hydroxylase (pan-TH) and...
Western Blotting (WB) image for anti-tyrosine Hydroxylase (TH) (pSer40) antibody (ABIN361503) Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolab...
Immunostaining (ISt) image for anti-tyrosine Hydroxylase (TH) (pSer40) antibody (ABIN361503) Immunostaining of light-stimulated rabbit retina showing labeling of TH when phosphor...
Produit citée dans: Calipari, Ferris, Melchior, Bermejo, Salahpour, Roberts, Jones: "Methylphenidate and cocaine self-administration produce distinct dopamine terminal alterations." dans: Addiction biology, Vol. 19, Issue 2, pp. 145-55, 2014 (PubMed).

Izumi, Ezumi, Takada-Takatori, Akaike, Kume: "Endogenous dopamine is involved in the herbicide paraquat-induced dopaminergic cell death." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 139, Issue 2, pp. 466-78, 2014 (PubMed).

Salvatore, Pruett: "Dichotomy of Tyrosine Hydroxylase and Dopamine Regulation between Somatodendritic and Terminal Field Areas of Nigrostriatal and Mesoaccumbens Pathways." dans: PLoS ONE, Vol. 7, Issue 1, pp. e29867, 2012 (PubMed).

McCutcheon, Conrad, Carr, Ford, McGehee, Marinelli: "Dopamine neurons in the ventral tegmental area fire faster in adolescent rats than in adults." dans: Journal of neurophysiology, Vol. 108, Issue 6, pp. 1620-30, 2012 (PubMed).

Nakashima, Mori, Kaneko, Hayashi, Nagatsu, Ota: "Phosphorylation of the N-terminal portion of tyrosine hydroxylase triggers proteasomal digestion of the enzyme." dans: Biochemical and biophysical research communications, Vol. 407, Issue 2, pp. 343-7, 2011 (PubMed). (1:3000).

Meyer, Richer, Benkovic, Hayashi, Kansy, Hale, Moy, Kim, OCallaghan, Tsai, Greengard, Nairn, Cowan, Miller, Antich, Bibb: "Striatal dysregulation of Cdk5 alters locomotor responses to cocaine, motor learning, and dendritic morphology." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18561-6, 2008 (PubMed).

Jedynak, Ali, Haycock, Hope: "Acute administration of cocaine regulates the phosphorylation of serine-19, -31 and -40 in tyrosine hydroxylase." dans: Journal of neurochemistry, Vol. 82, Issue 2, pp. 382-8, 2002 (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." dans: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, 2001 (PubMed).

Background publications Dunkley, Bobrovskaya, Graham, von Nagy-Felsobuki, Dickson: "Tyrosine hydroxylase phosphorylation: regulation and consequences." dans: Journal of neurochemistry, Vol. 91, Issue 5, pp. 1025-43, 2004 (PubMed).

Kish, Kalasinsky, Derkach, Schmunk, Guttman, Ang, Adams, Furukawa, Haycock: "Striatal dopaminergic and serotonergic markers in human heroin users." dans: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 24, Issue 5, pp. 561-7, 2001 (PubMed).

Witkovsky, Gabriel, Haycock, Meller: "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina." dans: Journal of chemical neuroanatomy, Vol. 19, Issue 2, pp. 105-16, 2000 (PubMed).

Zhu, Klimek, Haycock, Ordway: "Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain." dans: Journal of neuroscience methods, Vol. 99, Issue 1-2, pp. 37-44, 2000 (PubMed).

Zhu, Klimek, Dilley, Haycock, Stockmeier, Overholser, Meltzer, Ordway: "Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression." dans: Biological psychiatry, Vol. 46, Issue 9, pp. 1275-86, 1999 (PubMed).

Haycock, Ahn, Cobb, Krebs: "ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 6, pp. 2365-9, 1992 (PubMed).

Haycock, Bennett, George, Waymire: "Multiple site phosphorylation of tyrosine hydroxylase. Differential regulation in situ by a 8-bromo-cAMP and acetylcholine." dans: The Journal of biological chemistry, Vol. 257, Issue 22, pp. 13699-703, 1983 (PubMed).

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