ErbB2/Her2 anticorps (pTyr1248)

N° du produit ABIN362024

Détail du produit anti-ErbB2/Her2 anticorps

Antigène: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Épitope: pTyr1248

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ErbB2/Her2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificité: The antibody detects endogenous level of HER2 only when phosphorylated at tyrosine1248.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pTyr1248 (P-E-Y (p) -L-G) derived from Human HER2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-ErbB2/Her2 (ABIN362024)

Yuan, Qian, Shi, Lu, Li, Hu, Yu, Shen, Guo: "HER2-dependent MMP-7 expression is mediated by activated STAT3." dans: Cellular signalling, Vol. 20, Issue 7, pp. 1284-91, (2008) (PubMed).

Détails sur ErbB2/Her2

Antigène: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Autre désignation: HER2 (ErbB2/Her2 Produits)

Synonymes: anticorps CD340, anticorps HER-2, anticorps HER-2/neu, anticorps HER2, anticorps MLN 19, anticorps NEU, anticorps NGL, anticorps TKR1, anticorps Erbb-2, anticorps Neu, anticorps c-erbB2, anticorps c-neu, anticorps mKIAA3023, anticorps wu:fv70f10, anticorps zgc:63601, anticorps erb2, anticorps erb-b2 receptor tyrosine kinase 2, anticorps ERBB2, anticorps Erbb2, anticorps erbb2

Sujet: Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.

Poids moléculaire: 185 kDa

NCBI Accession: NP_001005

UniProt: P04626

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development