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Souris anti-Humain IgE (Fc Region) Anticorps

ELISA, WB, FACS, Func Monoclonal Le27-NBS01 IgG1 kappa unconjugated
N° du produit ABIN363695
  • Antigène Tous les produits IgE
    IgE
    Épitope
    • 30
    • 19
    • 17
    • 1
    • 1
    Fc Region
    Reactivité
    • 51
    • 31
    • 10
    • 10
    • 5
    • 4
    • 2
    • 1
    Humain
    Hôte
    • 58
    • 21
    • 11
    • 10
    • 4
    • 1
    Souris
    Clonalité
    • 66
    • 32
    Monoclonal
    Application
    • 55
    • 40
    • 30
    • 22
    • 20
    • 15
    • 12
    • 10
    • 10
    • 8
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
    Specificité
    Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes
    Attributs du produit
    IGHE, chromosome 14q32.33
    Purification
    Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
    Pureté
    > 95 %, tested by analytical Gel Permeation Chromatography
    Stérilité
    Sterile filtered
    Immunogène
    purified human myeloma IgE
    Clone
    Le27-NBS01
    Isotype
    IgG1 kappa
  • Indications d'application
    For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions less than 1:100 Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb Le27), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    PBS, pH=7.2, sterile filtered, contains no NaN3
    Agent conservateur
    Azide free
    Conseil sur la manipulation
    Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
    Stock
    4 °C
  • Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).

  • Antigène
    IgE
    Abstract
    IgE Produits
    Sujet
    The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.
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