Applications: Quality tested applications include - ELISA , FLISA FC ,
Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , WB , IP , Multiplex , Stim , Purification , Depletion
Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
0.5 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze! Protect conjugated products from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Stock
4 °C
Stockage commentaire
Store at 2-8°C
Ezzelarab, Zhang, Guo, Lu, Zahorchak, Wiseman, Nalesnik, Bhama, Cooper, Thomson: "Regulatory T Cell Infusion Can Enhance Memory T Cell and Alloantibody Responses in Lymphodepleted Nonhuman Primate Heart Allograft Recipients." dans: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, Vol. 16, Issue 7, pp. 1999-2015, (2017) (PubMed).
Azimzadeh, Byrne, Ezzelarab, Welty, Braileanu, Cheng, Robson, McGregor, Cooper, Pierson: "Development of a consensus protocol to quantify primate anti-non-Gal xenoreactive antibodies using pig aortic endothelial cells." dans: Xenotransplantation, Vol. 21, Issue 6, pp. 555-66, (2015) (PubMed).