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Souris anti-Humain IgG (Fc Region) Anticorps (PE)

ELISA, FACS Monoclonal JDC-10 IgG1 PE
N° du produit ABIN376566
  • Antigène Tous les produits IgG
    IgG
    Épitope
    • 2333
    • 498
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Fc Region
    Reactivité
    • 695
    • 671
    • 561
    • 458
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 97
    • 95
    • 92
    • 62
    • 47
    • 38
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 1730
    • 1474
    • 539
    • 205
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Souris
    Clonalité
    • 3361
    • 166
    • 1
    Monoclonal
    Conjugué
    • 557
    • 409
    • 406
    • 368
    • 298
    • 276
    • 228
    • 121
    • 98
    • 67
    • 66
    • 62
    • 62
    • 52
    • 48
    • 47
    • 45
    • 44
    • 44
    • 41
    • 39
    • 34
    • 33
    • 33
    • 31
    • 31
    • 30
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 26
    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    • 15
    • 14
    • 10
    PE
    Application
    • 1839
    • 1799
    • 1792
    • 1501
    • 962
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 52
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Flow Cytometry (FACS)
    Specificité
    Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA,
    Attributs du produit
    Mouse Anti-Human IgG Fc-PE
    Purification
    Purified
    Immunogène
    Purified human IgG1 myeloma protein
    Clone
    JDC-10
    Isotype
    IgG1
  • Indications d'application
    • Applications: ELISA - Quality tested , FLISA - Quality tested FC - Quality tested , IHC-PS - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature
    • Working Dilutions: ELISA AP conjugate 1:1,000 - 1:4,000 HRP conjugate 1:2,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA AF488 conjugate 1:200 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry Purified (UNLB) antibody 1 g/106 cells BIOT and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze!
    Protect conjugated products from light.

    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C
  • Gilman, Furmanova-Hollenstein, Pascual, B van t Wout, Langedijk, McLellan: "Transient opening of trimeric prefusion RSV F proteins." dans: Nature communications, Vol. 10, Issue 1, pp. 2105, (2019) (PubMed).

    Chung, Nguyen, Lin, Lafrance-Vanasse, Scales, Lin, Deng, Williams, Sperinde, Li, Zheng, Sukumaran, Tesar, Ernst, Fischer, Lazar, Prabhu, Song: "An in vitro FcRn- dependent transcytosis assay as a screening tool for predictive assessment of nonspecific clearance of antibody therapeutics in humans." dans: mAbs, Vol. 11, Issue 5, pp. 942-955, (2019) (PubMed).

    Boesch, Kappel, Mahan, Chu, Crowley, Osei-Owusu, Alter, Ackerman: "Enrichment of high affinity subclasses and glycoforms from serum-derived IgG using FcγRs as affinity ligands." dans: Biotechnology and bioengineering, Vol. 115, Issue 5, pp. 1265-1278, (2019) (PubMed).

    Brown, Dowell, Boesch, Normandin, Mahan, Chu, Barouch, Bailey-Kellogg, Alter, Ackerman: "Multiplexed Fc array for evaluation of antigen-specific antibody effector profiles." dans: Journal of immunological methods, Vol. 443, pp. 33-44, (2017) (PubMed).

    Brown, Normandin, Osei-Owusu, Mahan, Chan, Lai, Vaccari, Rao, Franchini, Alter, Ackerman: "Microscale purification of antigen-specific antibodies." dans: Journal of immunological methods, Vol. 425, pp. 27-36, (2016) (PubMed).

    Brown, Licht, Dugast, Choi, Bailey-Kellogg, Alter, Ackerman: "High-throughput, multiplexed IgG subclassing of antigen-specific antibodies from clinical samples." dans: Journal of immunological methods, Vol. 386, Issue 1-2, pp. 117-23, (2013) (PubMed).

  • Antigène
    IgG
    Abstract
    IgG Produits
    Classe de substances
    Antibody
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