MAP1LC3A anticorps (C-Term)

N° du produit ABIN388474

Détail du produit anti-MAP1LC3A anticorps

Antigène: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Épitope: AA 77-106, C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MAP1LC3A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène: This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the C-terminal region of human LC3.

Clone: RB7485

Isotype: Ig Fraction

Information d'application

Indications d'application: WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:50~100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 6 months

Références pour anticorps anti-MAP1LC3A (ABIN388474)

Eng, Panas, Karlsson Hedestam, McInerney: "A novel quantitative flow cytometry-based assay for autophagy." dans: Autophagy, Vol. 6, Issue 5, pp. 634-41, (2012) (PubMed).

Ryu, Kim, Yeo, Kang: "p65/RelA-Ser529 NF-?B subunit phosphorylation induces autophagic astroglial death (Clasmatodendrosis) following status epilepticus." dans: Cellular and molecular neurobiology, Vol. 31, Issue 7, pp. 1071-8, (2011) (PubMed).

Li, Wang, Fei, Xia, Qin, Liang: "Parkinson's disease involves autophagy and abnormal distribution of cathepsin L." dans: Neuroscience letters, Vol. 489, Issue 1, pp. 62-7, (2011) (PubMed).

Zhuang, Li, Long, Chen, Huang, Liang: "Knockdown of the DNA-dependent protein kinase catalytic subunit radiosensitizes glioma-initiating cells by inducing autophagy." dans: Brain research, Vol. 1371, pp. 7-15, (2011) (PubMed).

Bartlett, Isakson, Lewerenz, Sanchez, Kotzebue, Cumming, Harris, Nezis, Schubert, Simonsen, Finley: "p62, Ref(2)P and ubiquitinated proteins are conserved markers of neuronal aging, aggregate formation and progressive autophagic defects." dans: Autophagy, Vol. 7, Issue 6, pp. 572-83, (2011) (PubMed).

Arnold, Cassady, VanLaar, Berman: "Integrating multiple aspects of mitochondrial dynamics in neurons: age-related differences and dynamic changes in a chronic rotenone model." dans: Neurobiology of disease, Vol. 41, Issue 1, pp. 189-200, (2010) (PubMed).

Calakos, Patel, Gottron, Wang, Tran-Viet, Brewington, Beyer, Steffens, Krishnan, Züchner: "Functional evidence implicating a novel TOR1A mutation in idiopathic, late-onset focal dystonia." dans: Journal of medical genetics, Vol. 47, Issue 9, pp. 646-50, (2010) (PubMed).

Cherra, Kulich, Uechi, Balasubramani, Mountzouris, Day, Chu: "Regulation of the autophagy protein LC3 by phosphorylation." dans: The Journal of cell biology, Vol. 190, Issue 4, pp. 533-9, (2010) (PubMed).

Yang, Liu, Goga, Kim, Yuneva, Bishop: "Therapeutic potential of a synthetic lethal interaction between the MYC proto-oncogene and inhibition of aurora-B kinase." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 31, pp. 13836-41, (2010) (PubMed).

Wang, Gorvel, Chu, Wu, Lei: "Helicobacter pylori impairs murine dendritic cell responses to infection." dans: PLoS ONE, Vol. 5, Issue 5, pp. e10844, (2010) (PubMed).

Caramés, Taniguchi, Otsuki, Blanco, Lotz: "Autophagy is a protective mechanism in normal cartilage, and its aging-related loss is linked with cell death and osteoarthritis." dans: Arthritis and rheumatism, Vol. 62, Issue 3, pp. 791-801, (2010) (PubMed).

Ren, He, Nong, Zhu, Hu, Zhang, Huang, Zhu, Wu: "Acquired cisplatin resistance in human lung adenocarcinoma cells is associated with enhanced autophagy." dans: Cancer biotherapy & radiopharmaceuticals, Vol. 25, Issue 1, pp. 75-80, (2010) (PubMed).

Détails sur MAP1LC3A

Antigène: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Autre désignation: LC3 (MAP1LC3A Produits)

Synonymes: anticorps ATG8E, anticorps LC3, anticorps LC3A, anticorps MAP1ALC3, anticorps MAP1BLC3, anticorps 1010001H21Rik, anticorps 4922501H04Rik, anticorps LC3a, anticorps MGC69006, anticorps zgc:77094, anticorps MGC89867, anticorps MAP1LC3A, anticorps map1lc3a, anticorps microtubule associated protein 1 light chain 3 alpha, anticorps microtubule-associated protein 1 light chain 3 alpha, anticorps microtubule associated protein 1 light chain 3 alpha S homeolog, anticorps MAP1LC3A, anticorps Map1lc3a, anticorps map1lc3a.S, anticorps map1lc3a

Sujet: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Poids moléculaire: 14688

ID gène: 81631

NCBI Accession: NP_073729

UniProt: Q9GZQ8

Pathways: Autophagy