V5 Epitope Tag anticorps

N° du produit ABIN401225

Détail du produit anti-V5 Epitope Tag anticorps

Antigène: V5 Epitope Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp V5 Epitope Tag est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Séquence: GKPIPNPLLG LDST

Purification: Affinity chromatography

Immunogène: Peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1: 10,000 - 1: 60,000. Western blot: 1: 5,000 - 1: 10,000. Immunohistochemistry: 1: 500 - 1: 3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.

Détails sur V5 Epitope Tag

Antigène: V5 Epitope Tag

Autre désignation: Pk (V5) Epitope Tag (GKPIPNPLLGLDST) (V5 Epitope Tag Produits)

Classe de substances: Tag

Sujet: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.