ECM1 anticorps (Extracellular Matrix Protein 1)

Details for Product anti-ECM1 Antibody No. ABIN4306907, Fournisseur: Connectez-vous pour afficher
Antigène
  • URBWD
  • AI663821
  • p85
  • extracellular matrix protein 1
  • ECM1
  • Ecm1
Reactivité
Humain, Rat (Rattus)
95
63
21
1
1
1
1
1
1
1
1
Hôte
Souris
60
45
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp ECM1 est non-conjugé
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
ELISA, Electron Microscopy (EM), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
50
46
42
40
34
28
20
19
17
4
1
1
1
Options
Fournisseur
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N° du produit (Fournisseur)
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Immunogène Partially purified ECM1 from human colostrum was used as immunogen to generate the antibody (Bartek, 1989).
Clone SC05
Isotype IgG1 kappa
Specificité This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
Purification Protein G purified
Plasmids, Primers & others Plasmids, Primers & others ECM1 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation ECM-1/Secretory Component P85 (ECM1 Antibody Extrait)
Sujet Gene Symbol: ECM1
Poids moléculaire Theoretical MW: 80 kDa
ID gène 1893
UniProt Q16610
Indications d'application Flow Cytometry 0.5-1 μg/million cells, ELISA 1-5 μg/mL for coating, Immunohistochemistry 0.5-1 μg/mL, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunoprecipitation 1-2 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 0.5-1 μg/mL, Immunohistochemistry-Frozen 0.5-1 μg/mL, Electron MicroscopyStaining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer 10 mM PBS and 0.05 % BSA
Buffer contains: 0.05 % Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C
Stockage commentaire Store at 4°C.
Images (Fournisseur)
Immunofluorescence (IF) image for anti-Extracellular Matrix Protein 1 (ECM1) antibody (ABIN4306907) Immunocytochemistry/Immunofluorescence: ECM-1/Secretory Component P85 Antibody (SC05)...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Extracellular Matrix Protein 1 (ECM1) antibody (ABIN4306907) Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SC05) [NBP2-29...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Extracellular Matrix Protein 1 (ECM1) antibody (ABIN4306907) Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SC05) [NBP2-29...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Extracellular Matrix Protein 1 (ECM1) antibody (ABIN4306907) Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SC05) [NBP2-29...
Produit citée dans: Philley, Kannan, Griffith, Devine, Benwill, Wallace, Brown-Elliott, Thakkar, Taskar, Fox, Alqaid, Bains, Gupta, Dasgupta: "Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease." dans: Oncotarget, Vol. 8, Issue 11, pp. 18070-18081, 2017 (PubMed). Méthode utilisée: Flow Cytometry (FACS) (Échantillon (espèces): Human).

Polosukhin, Cates, Lawson, Zaynagetdinov, Milstone, Massion, Ocak, Ware, Lee, Bowler, Kononov, Randell, Blackwell: "Bronchial secretory immunoglobulin a deficiency correlates with airway inflammation and progression of chronic obstructive pulmonary disease." dans: American journal of respiratory and critical care medicine, Vol. 184, Issue 3, pp. 317-27, 2011 (PubMed). Détails: Immunohistochemistry (Paraffin-embedded Sections)

Gologan, Acquafondata, Dhir, Sepulveda: "Polymeric immunoglobulin receptor-negative tumors represent a more aggressive type of adenocarcinomas of distal esophagus and gastroesophageal junction." dans: Archives of pathology & laboratory medicine, Vol. 132, Issue 8, pp. 1295-301, 2008 (PubMed). Détails: Immunohistochemistry (Paraffin-embedded Sections)

Tang, Tao, Yun-Liu, Sun, Geng, Jiang: "Immunocytochemical localization of secretory component in Paneth cell secretory granules-rat Paneth cells participate in acquired immunity." dans: Journal of molecular histology, Vol. 36, Issue 5, pp. 331-5, 2005 (PubMed).

Fichorova, Anderson: "Differential expression of immunobiological mediators by immortalized human cervical and vaginal epithelial cells." dans: Biology of reproduction, Vol. 60, Issue 2, pp. 508-14, 1999 (PubMed). Détails: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Fichorova, Rheinwald, Anderson: "Generation of papillomavirus-immortalized cell lines from normal human ectocervical, endocervical, and vaginal epithelium that maintain expression of tissue-specific differentiation proteins." dans: Biology of reproduction, Vol. 57, Issue 4, pp. 847-55, 1997 (PubMed). Détails: Immunocytochemistry,Immunohistochemistry (Frozen Sections)

Bartek, Bartkova, Taylor-Papadimitriou: "Keratin 19 expression in the adult and developing human mammary gland." dans: The Histochemical journal, Vol. 22, Issue 10, pp. 537-44, 1991 (PubMed). (Échantillon (espèces): Human). Détails: Immunohistochemistry (Paraffin-embedded Sections)

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