Indications d'application | Western Blot 1:1000, Flow Cytometry 1 μg/ 10^6 cells, Immunohistochemistry 1:100, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1 μg/mL, Immunohistochemistry-Frozen 1:100, Immunofluorescence 1:100 - 1:200, Immunocytochemistry 1:250WB: Detects an approx. 110 kDa protein representing SERCA2 ATPase in rat cardiac tissue. Used in IHC-Fr. |
Commentaires |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Restrictions | For Research Use only |
Format | Liquid |
Buffer |
Ascites diluted with PBS Buffer contains: 0.05 % Sodium Azide |
Agent conservateur | Sodium azide |
Précaution d'utilisation | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Conseil sur la manipulation | Avoid freeze-thaw cycles |
Stock | -20 °C |
Stockage commentaire | Store at -20°C. Avoid freeze-thaw cycles. |
Images (Fournisseur) |
|
Produit citée dans: |
Klug, Boule, Wissocque, Montaigne, Marechal, Hassoun, Neviere: "Right ventricular pacing with mechanical dyssynchrony causes apoptosis interruptus and calcium mishandling." dans: The Canadian journal of cardiology, Vol. 29, Issue 4, pp. 510-8, 2013 (PubMed).
(Échantillon (espèces): Pig (Porcine)). Détails: Western Blotting Schroeder, Ali, Hulikova, Supuran, Clarke, Vaughan-Jones, Tyler, Swietach: "Extramitochondrial domain rich in carbonic anhydrase activity improves myocardial energetics." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 10, pp. E958-67, 2013 (PubMed). (Échantillon (espèces): Rat (Rattus)). Détails: Western Blotting García-Pérez, Hajnóczky, Csordás: "Physical coupling supports the local Ca2+ transfer between sarcoplasmic reticulum subdomains and the mitochondria in heart muscle." dans: The Journal of biological chemistry, Vol. 283, Issue 47, pp. 32771-80, 2008 (PubMed). Détails: Immunohistochemistry Prasad, Ma, Sumbilla, Lee, Klein, Inesi: "Phenylephrine hypertrophy, Ca2+-ATPase (SERCA2), and Ca2+ signaling in neonatal rat cardiac myocytes." dans: American journal of physiology. Cell physiology, Vol. 292, Issue 6, pp. C2269-75, 2007 (PubMed). Détails: Immunocytochemistry,Immunofluorescence Anyatonwu, Estrada, Tian, Somlo, Ehrlich: "Regulation of ryanodine receptor-dependent calcium signaling by polycystin-2." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 15, pp. 6454-9, 2007 (PubMed). Détails: Immunoprecipitation Khan, Lee, Minhas, Gonzalez, Raju, Tejani, Li, Berkowitz, Hare: "Neuronal nitric oxide synthase negatively regulates xanthine oxidoreductase inhibition of cardiac excitation-contraction coupling." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 45, pp. 15944-8, 2004 (PubMed). Détails: Immunoprecipitation Krizaj, Liu, Copenhagen: "Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum)." dans: The Journal of comparative neurology, Vol. 475, Issue 4, pp. 463-80, 2004 (PubMed). Détails: Immunohistochemistry Spragg, Leclercq, Loghmani, Faris, Tunin, DiSilvestre, McVeigh, Tomaselli, Kass: "Regional alterations in protein expression in the dyssynchronous failing heart." dans: Circulation, Vol. 108, Issue 8, pp. 929-32, 2003 (PubMed). Détails: Western Blotting Asahi, Kurzydlowski, Tada, MacLennan: "Sarcolipin inhibits polymerization of phospholamban to induce superinhibition of sarco(endo)plasmic reticulum Ca2+-ATPases (SERCAs)." dans: The Journal of biological chemistry, Vol. 277, Issue 30, pp. 26725-8, 2002 (PubMed). Détails: Immunoprecipitation Tupling, Asahi, MacLennan: "Sarcolipin overexpression in rat slow twitch muscle inhibits sarcoplasmic reticulum Ca2+ uptake and impairs contractile function." dans: The Journal of biological chemistry, Vol. 277, Issue 47, pp. 44740-6, 2002 (PubMed). Détails: Immunocytochemistry,Immunofluorescence Movsesian, Karimi, Green, Jones: "Ca(2+)-transporting ATPase, phospholamban, and calsequestrin levels in nonfailing and failing human myocardium." dans: Circulation, Vol. 90, Issue 2, pp. 653-7, 1994 (PubMed). Détails: Western Blotting |