Mouton anti-Chien IgG (Fc Region) Anticorps

N° du produit ABIN457675

Détail du produit

Antigène: IgG

Épitope: Fc Region

Reactivité: Chien

Hôte: Mouton

Clonalité: Polyclonal

Application: Immunoprecipitation (IP)

Specificité: Precipitating polyclonal Sheep antiSerum to Dog IgG, Fc specific.

Réactivité croisée (Details): The antiSerum does not cross-react with any other component of the Dog immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Attributs du produit: The reactivity of the antiserum is restricted to the Fc part of the IgG molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal dog serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No precipitation reaction is obtained with purified IgA, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in dog serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of dog IgG from serum or plasma. The antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive techniques. For such uses labelled and unlabelled cytochemical grade reagents raised in goats meeting the necessary additional specificity and performance requirements are available.

Purification: Delipidated, heat inactivated, stable whole serum

Stérilité: Heat-inactivated

Immunogène: Highly purified normal IgG isolated from pooled dog serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: In immunoelectrophoresis use 2 μL serum or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in 3 mm diameter center well and 2 μL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.Delipidated, heat inactivated, lyophilized, stable whole antiserum. 0.01 % sodium azide. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal sheep serum. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum. 0.01 % sodium azide. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal sheep serum. No foreign proteins added.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody