Mouton anti-Souris IgG (Chain kappa), (Chain lambda), (Fab Region), (Free & Bound) Anticorps

N° du produit ABIN458478

Détail du produit

Antigène: IgG

Épitope: Chain kappa, Chain lambda, Fab Region, Free & Bound

Reactivité: Souris

Hôte: Mouton

Clonalité: Polyclonal

Application: Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Specificité: The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion

Attributs du produit: Precipitating polyclonal sheep antiserum to mouse Fab of IgG
Total protein and IgG concentration in the antiserum are comparable to those of pooled sheep serum. Antibody titre: Precipitin titre not less than 1:64 when tested against normal mouse serum in agar block titration.

Purification: Delipidated, heat inactivated lyophilized stable whole serum
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies reacting with other subunits of the immunoglobulin or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of foreign protein or immune complexes in the antiserum.

Immunogène: Purified Fab of normal mouse IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: In precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of Fab, free or bound.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water.

Buffer: No foreign proteins added.

Agent conservateur: Without preservative

Conseil sur la manipulation: Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody