Lapin anti-Mouton IgM (Fab Region), (Fc Region) Anticorps (HRP) - Preadsorbed

Détails pour le produit réf. ABIN458777
Antigène
Épitope
Fc Region, Fab Region
103
82
49
36
19
6
1
1
Reactivité
Mouton
161
89
46
21
20
16
14
11
11
8
8
8
8
7
7
5
2
2
1
1
Hôte
Lapin
219
104
75
11
11
5
4
2
1
1
1
Clonalité
Polyclonal
Conjugué
HRP
53
47
43
25
23
20
17
9
7
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA
Options
Immunogène Purified normal IgM isolated from pooled sheep serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificité The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgM molecule.
Attributs du produit Horseradish peroxidase-conjugated IgG fraction of polyclonal rabbit antiserum to sheep IgM, heavy and light chains
Peroxidase/IgG protein molar ratio (E/P) is approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2).
Purification Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
Antigène
Indications d'application In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:10,000.
Restrictions For Research Use only
Format Lyophilized
Reconstitution It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
Concentration 10 mg/mL
Buffer Horseradish peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
Agent conservateur Without preservative
Conseil sur la manipulation Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Stock 4 °C/-20 °C
Stockage commentaire The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.