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LDH5 anticorps (Biotin)

LDH5 Reactivité: Porc WB, IF, ELISA Hôte: Lapin Polyclonal Biotin
N° du produit ABIN459062
  • Antigène Tous les produits LDH5
    LDH5 (Lactate Dehydrogenase Isoenzyme 5 (LDH5))
    Reactivité
    • 4
    • 4
    • 3
    Porc
    Hôte
    • 6
    • 5
    Lapin
    Clonalité
    • 11
    Polyclonal
    Conjugué
    • 7
    • 4
    Cet anticorp LDH5 est conjugé à/à la Biotin
    Application
    • 10
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 4
    Western Blotting (WB), Immunofluorescence (IF), ELISA
    Specificité
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    Attributs du produit
    Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to L-lactic dehydrogenase, LDH-1(M 4 ) isoenzyme, from porcine heart
    Purification
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    Immunogène
    L-Lactic dehydrogenase, LDH-1(M 4 ) isoenzyme, isolated and purified from porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Indications d'application
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.4. No foreign proteins added.
    Buffer
    Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
    Agent conservateur
    Without preservative
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slow ly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same da y. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Antigène
    LDH5 (Lactate Dehydrogenase Isoenzyme 5 (LDH5))
    Abstract
    LDH5 Produits
    Sujet
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immuno-diffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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