MMP 9 anticorps (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase))

Details for Product anti-MMP9 Antibody No. ABIN461805
Antigène
Reactivité
Humain, Rat (Rattus)
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Hôte
Souris
349
162
2
2
1
1
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp MMP 9 est non-conjugé
26
16
12
7
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5
5
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5
3
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Application
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
375
196
186
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72
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9
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Options
Marque IHC-plus™
Immunogène Human native MMP-9. MW of Antigen: Pro form: 92kD Active form: ~86kD. Cellular Localization: Cytoplasmic.

Type of Immunogen: Purified protein
Clone IIA5
Isotype IgG1, kappa
Specificité Recognizes pro (latent) and activated forms of matrix metalloproteinase 9. Species cross-reactivity: human and rat.
Purification Protein G purified
Antigène
Autre désignation MMP9 / Gelatinase B (MMP9 Antibody Extrait)
Sujet Name/Gene ID: MMP9
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP9, 92kd gelatinase, 92 kd type iv collagenase, 92 kDa gelatinase, 92 kDa type IV collagenase, Matrix metalloproteinase-9, MMP-9, Type V collagenase, CLG4B, Gelatinase B, GELB, Macrophage gelatinase, MANDP2
ID gène 4318
UniProt P14780
Pathways Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events
Indications d'application Approved: ELISA, IHC, IHC-P (1:50), IP, WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. Western Blot: 1-2 μg/mL for 2 hrs at RT. Immunoprecipitation: Native. Positive control: Conditioned, serum-free medium from (TPA-treated) human fibrosarcoma HT-1080 or CCL-4 cells.
Commentaires

Target Species of Antibody: Human

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS  pH 7.4 with 0.2 % BSA and 0.09 % sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid repeat freeze-thaw cycles.
Stock 4 °C,-20 °C
Stockage commentaire Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Images (Fournisseur)
Image no. 1 for anti-Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9) antibody (ABIN461805) Human Spleen (formalin-fixed, paraffin-embedded) stained with MMP9 antibody ABIN46180...