SRC anticorps (V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian)) (AA 92-109)

Details for Product anti-SRC Antibody No. ABIN461967
Antigène
Épitope
AA 92-109
69
43
42
38
35
20
19
18
12
12
10
10
7
7
7
5
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Roussette (Chauve-souris), Poulet, Boeuf (Vache), Chien, Hamster, Cheval, Humain, Singe, Souris, Porc, Lapin, Rat (Rattus), Xenopus laevis
515
320
287
21
15
14
14
10
10
5
4
4
4
3
2
1
1
1
1
Hôte
Lapin
473
63
1
Clonalité
Polyclonal
Conjugué
Cet anticorp SRC est non-conjugé
29
24
19
15
15
14
11
11
11
11
11
11
5
5
5
5
5
2
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
405
246
187
67
66
43
35
25
19
11
10
6
5
3
1
Options
Marque IHC-plus™
Immunogène An 18 residue synthetic peptide YDYESRTETDLSFKKGER based on the sequence from the SH3 domain of human Src (residues 92-109) was synthesized and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Xenopus (100%), Lizard, Stickleback, Pufferfish, Zebrafish (83%).

Type of Immunogen: Synthetic peptide - KLH conjugated
Specificité Detects an ~60 kD band, corresponding to the apparent molecular mass of Src on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, chicken, fish (rainbow trout), guinea pig, hamster, monkey, pig, rabbit, sheep and Xenopus origins.
Homologie Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Xenopus (100%) Lizard, Stickleback, Pufferfish, Zebrafish (83%).
Purification Antiserum
Antigène
Autre désignation SRC (SRC Antibody Extrait)
Sujet Name/Gene ID: SRC
Subfamily: Src
Family: Protein Kinase

Synonyms: SRC, ASV, Pp60c-src, Tyrosine kinase pp60c-src, Tyrosine-protein kinase SRC-1, v-src, C-SRC, p60-Src, Proto-oncogene c-Src, SRC1
ID gène 6714
UniProt P12931
Pathways Signalistation JAK/STAT, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
Indications d'application Approved: IHC, IHC-P (1:250), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:250.
Commentaires

Target Species of Antibody: Human

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Whole antisera
Conseil sur la manipulation Avoid repeated freezing and thawing.
Stock 4 °C,-20 °C
Stockage commentaire 4°C or -20°C, Avoid freeze-thaw cycles.
Images (Fournisseur)
Image no. 1 for anti-V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) (AA 92-109) antibody (ABIN461967) Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with SRC antibody...
Image no. 2 for anti-V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) (AA 92-109) antibody (ABIN461967) Anti-SRC antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fi...