BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3) (AA 1-316) anticorps

Détails pour le produit réf. ABIN462274
Antigène
Épitope
AA 1-316
14
12
4
4
2
2
1
1
1
1
Reactivité
Humain
95
43
42
6
5
3
3
3
3
2
1
1
Hôte
Souris
78
17
Clonalité (Clone)
Monoclonal ()
Conjugué
Inconjugué
6
4
4
3
3
3
3
3
3
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
67
34
24
21
13
7
3
3
2
2
1
1
Options
Marque IHC-plus™
Immunogène Recombinant BRCC36 (1-316aa) purified from E. coli

Type of Immunogen: Recombinant protein
Clone AT3B1
Isotype IgG1, kappa
Specificité BRCC36 (1-316aa)
Purification Protein G purified
Antigène
Autre désignation BRCC3 / BRCC36 (BRCC3 Antibody Extrait)
Sujet Name/Gene ID: BRCC3

Synonyms: BRCC3, C6.1A, BRCC36, BRISC complex subunit BRCC36, BRCA1-A complex subunit BRCC36, CXorf53, RP11-143H17.2
ID gène 79184
UniProt P46736
Pathways Positive Regulation of Response to DNA Damage Stimulus
Indications d'application Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Cell lysates of HeLa(20 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Commentaires

Target Species of Antibody: Human

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS,  pH 7.4, 0.1 % sodium azide. Sourced in Ascites.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Minimize freezing and thawing.
Stock -20 °C
Stockage commentaire Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Images (Fournisseur)
Image no. 1 for anti-BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3) (AA 1-316) antibody (ABIN462274) Human Kidney (formalin-fixed, paraffin-embedded) stained with antibody ABIN462274 fo...
Image no. 2 for anti-BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3) (AA 1-316) antibody (ABIN462274) Human Spleen (formalin-fixed, paraffin-embedded) stained with antibody ABIN462274 fo...