GNAO1 anticorps (Guanine Nucleotide Binding Protein (G Protein), alpha Activating Activity Polypeptide O) (AA 345-354)

Details for Product anti-GNAO1 Antibody No. ABIN462341
Antigène
Épitope
AA 345-354, Subunit alpha
10
9
7
4
2
2
1
1
1
1
1
1
Reactivité
Mouche des fruits (Drosophila melanogaster), Souris
77
70
39
4
4
4
4
3
2
2
2
1
1
1
1
1
Hôte
Lapin
90
1
Clonalité
Polyclonal
Conjugué
Cet anticorp GNAO1 est non-conjugé
9
6
6
4
4
4
4
4
4
2
2
2
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
59
40
19
17
13
6
2
1
Options
Marque IHC-plus™
Immunogène Synthetic peptide from mouse GNAO1 / GNAO.

Type of Immunogen: Synthetic peptide
Isotype IgG
Specificité Recognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40kD forming a heterotrimeric protein complex with beta and gamma subunits. Two isoforms of the alpha subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2. Has been used successfully for the detection of G(o) in Drosophila by western blotting.
Purification Ammonium sulfate precipitation
Antigène
Autre désignation GNAO1 (GNAO1 Antibody Extrait)
Sujet Name/Gene ID: GNAO1

Synonyms: GNAO1, GNAO, G-ALPHA-o, G0 Protein Alpha
ID gène 2775
UniProt P09471
Pathways G-protein mediated Events
Indications d'application Approved: ELISA (1:500 - 1:1000), IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of formalin-fixed, paraffin-embedded (FFPE) mouse tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: This antibody detects band at 39 kD when used under reducing conditions against rat brain.
Commentaires

Target Species of Antibody: Mouse

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, 0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid repeated freezing and thawing.
Stock 4 °C,-20 °C
Stockage commentaire 4°C or -20°C, Avoid freeze-thaw cycles.
Images (Fournisseur)
Image no. 1 for anti-Guanine Nucleotide Binding Protein (G Protein), alpha Activating Activity Polypeptide O (GNAO1) (AA 345-354), (Subunit alpha) antibody (ABIN462341) Anti-GNAO1 antibody IHC of mouse brain, cerebellum. Immunohistochemistry of formalin-...
Image no. 2 for anti-Guanine Nucleotide Binding Protein (G Protein), alpha Activating Activity Polypeptide O (GNAO1) (AA 345-354), (Subunit alpha) antibody (ABIN462341) Mouse Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with GNAO1 antibo...
Image no. 3 for anti-Guanine Nucleotide Binding Protein (G Protein), alpha Activating Activity Polypeptide O (GNAO1) (AA 345-354), (Subunit alpha) antibody (ABIN462341) Mouse Brain, Cortex (formalin-fixed, paraffin-embedded) stained with GNAO1 antibody A...