M-CSF/CSF1 anticorps

N° du produit ABIN465551

Détail du produit anti-M-CSF/CSF1 anticorps

Antigène: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp M-CSF/CSF1 est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificité: Human M-CSF

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human M-CSF.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA, Neut (2 - 3 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hM-CSF (7 ng/mL), a concentration of 0.02-0.03 μg/mL of this antibody is required. ELISA: To detect hM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human M-CSF (LS-C104655) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hM-CSF. Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur M-CSF/CSF1

Antigène: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Autre désignation: CSF1 / CSF-1 / MCSF (CSF1 Produits)

Synonymes: anticorps CSF-1, anticorps MCSF, anticorps C87615, anticorps Csfm, anticorps op, anticorps csf1-1, anticorps zgc:172186, anticorps CSF1, anticorps csf1-2, anticorps zgc:158436, anticorps colony stimulating factor 1, anticorps colony stimulating factor 1 (macrophage), anticorps colony stimulating factor 1a (macrophage), anticorps macrophage colony-stimulating factor 1, anticorps macrophage colony stimulating factor, anticorps colony stimulating factor 1b (macrophage), anticorps CSF1, anticorps Csf1, anticorps csf1a, anticorps LOC396599, anticorps LOC100860895, anticorps csf1b

Sujet: Name/Gene ID: CSF1
Family: Hormone

Synonyms: CSF1, CSF-1, MCSF, M-CSF, Lanimostim

ID gène: 1435

UniProt: P09603

Pathways: Signalisation RTK