CCL2 anticorps
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- Antigène Voir toutes CCL2 Anticorps
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
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Reactivité
- Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp CCL2 est non-conjugé
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Specificité
- Rat MCP-1
- Purification
- Immunoaffinity purified
- Immunogène
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E.coli derived recombinant Rat MCP-1.
Type of Immunogen: Cell extract - Top Product
- Discover our top product CCL2 Anticorps primaire
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- Indications d'application
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Approved: ELISA (0.5 - 2 μg/mL), Neut (4 - 6 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained colchicine injected rat brain (including the caudate putamen) tissue. Frozen samples fixed in 4 % PFA. The primary antibody was incubated at 1 μg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat MCP-1 (MCAF) (100 ng/mL), a concentration of 4-6 μg/mL of this antibody is required. ELISA: To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat MCP-1(MCAF) (LS-C104762) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MCP-1(MCAF). Western Blot: To detect Rat MCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1 (MCAF) is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Commentaires
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Target Species of Antibody: Rat
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Conseil sur la manipulation
- Avoid freeze/thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Antigène
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
- Autre désignation
- CCL2 / MCP-1 (CCL2 Produits)
- Synonymes
- anticorps GDCF-2, anticorps HC11, anticorps HSMCR30, anticorps MCAF, anticorps MCP-1, anticorps MCP1, anticorps SCYA2, anticorps SMC-CF, anticorps AI323594, anticorps JE, anticorps Scya2, anticorps Sigje, anticorps MCP-1A, anticorps MCP1A, anticorps C-C motif chemokine ligand 2, anticorps chemokine (C-C motif) ligand 2, anticorps C-C motif chemokine 2, anticorps CCL2, anticorps Ccl2, anticorps LOC101120093
- Sujet
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Name/Gene ID: CCL2
Subfamily: Intercrine beta
Family: Intercrine
Synonyms: CCL2, C-C motif chemokine 2, Chemokine (C-C motif) ligand 2, GDCF-2, HC11, HSMCR30, MCP1, SCYA2, Small-inducible cytokine A2, Monocyte secretory protein JE, MCAF, MCP-1, Monocyte chemotactic protein 1, SMC-CF - ID gène
- 6347
- UniProt
- P13500
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylation & l'infection par le SRAS-CoV-2
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